Recombinant Human HEXB protein (ab198424)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 82% SDS-PAGE
- Tags: DDDDK tag C-Terminus
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant Human HEXB protein
See all HEXB proteins and peptides -
Purity
> 82 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MELCGLGLPRPPMLLALLLATLLAAMLALLTQVALVVQVAEAARAPSVSA KPGPALWP LPLSVKMTPNLLHLAPENFYISHSPNSTAGPSCTLLEEAF RRYHGYIFGFYKWHHEPAE FQAKTQVQQLLVSITLQSECDAFPNISSD ESYTLLVKEPVAVLKANRVWGALRGLETFS QLVYQDSYGTFTINESTI IDSPRFSHRGILIDTSRHYLPVKIILKTLDAMAFNKFNVLHWHIV DDQ SFPYQSITFPELSNKGSYSLSHVYTPNDVRMVIEYARLRGIRVLPEFDTP GHTLSWG KGQKDLLTPCYSRQNKLDSFGPINPTLNTTYSFLTTFFKEI SEVFPDQFIHLGGDEVEFK CWESNPKIQDFMRQKGFGTDFKKLESFYI QKVLDIIATINKGSIVWQEVFDDKAKLAPGTI VEVWKDSAYPEELSRV TASGFPVILSAPWYLDLISYGQDWRKYYKVEPLDFGGTQKQK QLFIGG EACLWGEYVDATNLTPRLWPRASAVGERLWSSKDVRDMDDAYDRLTRHRC R MVERGIAAQPLYAGYCNHENMDYKDDDDKHHHHHH -
Predicted molecular weight
65 kDa including tags -
Tags
DDDDK tag C-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab198424 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Preservative: 1.02% Imidazole
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 20% Glycerol
General Info
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Alternative names
- Beta hexosaminidase beta chain
- Beta hexosaminidase subunit beta
- Beta N acetylhexosaminidase
see all -
Function
Responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues. -
Involvement in disease
Defects in HEXB are the cause of GM2-gangliosidosis type 2 (GM2G2) [MIM:268800]; also known as Sandhoff disease. GM2-gangliosidosis is an autosomal recessive lysosomal storage disease marked by the accumulation of GM2 gangliosides in the neuronal cells. GM2G2 is clinically indistinguishable from GM2-gangliosidosis type 1, presenting startle reactions, early blindness, progressive motor and mental deterioration, macrocephaly and cherry-red spots on the macula. -
Sequence similarities
Belongs to the glycosyl hydrolase 20 family. -
Post-translational
modificationsN-linked glycans at Asn-142 and Asn-190 consist of Man(3)-GlcNAc(2) and Man(5 to 7)-GlcNAc(2), respectively.
The beta-A and beta-B chains are produced by proteolytic processing of the precursor beta chain. -
Cellular localization
Lysosome. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab198424 has not yet been referenced specifically in any publications.