Key features and details
- Expression system: HEK 293 cells
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, HPLC, Functional Studies
Product nameRecombinant human HGF protein (Active)
See all HGF proteins and peptides
Determined by the dose-dependent stimulation of the proliferation of monkey 4MBr-5 cells.
Endotoxin level< 1.000 Eu/µg
Expression systemHEK 293 cells
Protein lengthFull length protein
Sequencealpha chain: KRRNTIHEFK KSAKTTLIKI DPALKIKTKK VNTADQCANR CTRNKGLPFT CKAFVFDKAR KQCLWFPFNS MSSGVKKEFG HEFDLYENKD YIRNCIIGKG RSYKGTVSIT KSGIKCQPWS SMIPHEHSFL PSSYRGKDLQ ENYCRNPRGE EGGPWCFTSN PEVRYEVCDI PQCSEVECMT CNGESYRGLM DHTESGKICQ RWDHQTPHRH KFLPERYPDK GFDDNYCRNP DGQPRPWCYT LDPHTRWEYC AIKTCADNTM NDTDVPLETT ECIQGQGEGY RGTVNTIWNG IPCQRWDSQY PHEHDMTPEN FKCKDLRENY CRNPDGSESP WCFTTDPNIR VGYCSQIPNC DMSHGQDCYR GNGKNYMGNL SQTRSGLTCS MWDKNMEDLH RHIFWEPDAS KLNENYCRNP DDDAHGPWCY TGNPLIPWDY CPISRCEGDT TPTIVNLDHP VISCAKTKQL R beta chain: VVNGIPTRTN IGWMVSLRYR NKHICGGSLI KESWVLTARQ CFPSRDLKDY EAWLGIHDVH GRGDEKCKQV LNVSQLVYGP EGSDLVLMKL ARPAVLDDFV STIDLPNYGC TIPEKTSCSV YGWGYTGLIN YDGLLRVAHL YIMGNEKCSQ HHRGKVTLNE SEICAGAEKI GSGPCEGDYG GPLVCEQHKM RMVLGVIVPG RGCAIPNRPG IFVRVAYYAK WIHKIILTYK VPQS
Predicted molecular weight79 kDa
Amino acids33 to 728
Additional sequence informationFull length protein without the signal peptide. consiting of the alpha chain (32-494aa) and the beta chain (495-728aa)
Our Abpromise guarantee covers the use of ab245957 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
As a result of glycosylation, ab245957 migrates with an apparent molecular mass of approximately 68-85 kDa by SDS-PAGE gel, under non-reducing conditions.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionReconstitute in water to 0.1 - 1.0 mg/ml.
- F TCF
- Fibroblast derived tumor cytotoxic factor
FunctionPotent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization.
Involvement in diseaseDeafness, autosomal recessive, 39
Sequence similaritiesBelongs to the peptidase S1 family. Plasminogen subfamily.
Contains 4 kringle domains.
Contains 1 PAN domain.
Contains 1 peptidase S1 domain.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245957 has not yet been referenced specifically in any publications.