Recombinant Human HINT1 protein (ab87362)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant Human HINT1 protein
See all HINT1 proteins and peptides -
Purity
> 90 % SDS-PAGE.
Purified by using conventional chromatography. -
Expression system
Escherichia coli -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MADEIAKAQV ARPGGDTIFG KIIRKEIPAK IIFEDDRCLA FHDISPQAPT HFLVIPKKHI SQISVAEDDD ESLLGHLMIV GKKCAADLGL NKGYRMVVNE GSDGGQSVYH VHLHVLGGRQ MHWPPG
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Specifications
Our Abpromise guarantee covers the use of ab87362 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C.
pH: 8.00
Constituents: 0.316% Tris HCl, 10% Glycerol (glycerin, glycerine)
General Info
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Alternative names
- Adenosine 5' monophosphoramidase
- Adenosine 5''-monophosphoramidase
- HINT 1
see all -
Function
Hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the HINT family.
Contains 1 HIT domain. -
Domain
The histidine triad, also called HIT motif, forms part of the binding loop for the alpha-phosphate of purine mononucleotide. -
Cellular localization
Cytoplasm. Nucleus. Interaction with CDK7 leads to a more nuclear localization. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab87362 has been referenced in 1 publication.
- Cortés-Montero E et al. The Axonal Motor Neuropathy-Related HINT1 Protein Is a Zinc- and Calmodulin-Regulated Cysteine SUMO Protease. Antioxid Redox Signal 31:503-520 (2019). PubMed: 31088288