• Product name

    Recombinant Human HIP2/LIG protein
    See all HIP2/LIG proteins and peptides
  • Biological activity

    ab111465 charges and supports ubiquitinylation in vitro. Typical enzyme concentration to support conjugation in vitro is 100 nM to 1 µM
  • Purity

    > 95 % SDS-PAGE.

  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Full length protein
  • Animal free

  • Nature

    • Species

    • Predicted molecular weight

      22 kDa
    • Amino acids

      1 to 200


Our Abpromise guarantee covers the use of ab111465 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications


  • Form

  • Additional notes

    ab111465 charges and supports ubiquitinylation in vitro. Typical enzyme concentration to support conjugation in vitro is 100 nM to 1 µM

     This product was previously labelled as HIP2


  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: Tris buffered saline, 0.02% DTT

General Info

  • Alternative names

    • E2 25K
    • E2(25K)
    • HIP-2
    • Huntingtin interacting protein 2
    • Huntingtin-interacting protein 2
    • HYPG
    • LIG
    • UBC1
    • UBE2K
    • Ubiquitin carrier protein
    • Ubiquitin conjugating enzyme E2 25 kDa
    • ubiquitin conjugating enzyme E2K
    • ubiquitin conjugating enzyme E2K (UBC1 homolog, yeast)
    • Ubiquitin protein ligase
    • Ubiquitin-conjugating enzyme E2 K
    • Ubiquitin-conjugating enzyme E2(25K)
    • Ubiquitin-conjugating enzyme E2-25 kDa
    • Ubiquitin-conjugating enzyme E2-25K
    • Ubiquitin-protein ligase
    see all
  • Function

    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, in the presence or in the absence of BRCA1-BARD1 E3 ubiquitin-protein ligase complex, catalyzes the synthesis of 'Lys-48'-linked polyubiquitin chains. Does not transfer ubiquitin directly to but elongates monoubiquitinated substrate protein. Mediates the selective degradation of short-lived and abnormal proteins, such as the endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. Ubiquitinates huntingtin. May mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination and subsequence degradation of p53/TP53. Proposed to be involved in ubiquitination and proteolytic processing of NF-kappa-B; in vitro supports ubiquitination of NFKB1. In case of infection by cytomegaloviruses may be involved in the US11-dependent degradation of MHC class I heavy chains following their export from the ER to the cytosol. In case of viral infections may be involved in the HPV E7 protein-dependent degradation of RB1.
  • Tissue specificity

    Expressed in all tissues tested, including spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocytes, T-lymphocytes, monocytes, granulocytes and bone marrow mononuclear cells. Highly expressed in brain, with highest levels found in cortex and striatum and at lower levels in cerebellum and brainstem.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the ubiquitin-conjugating enzyme family.
    Contains 1 UBA domain.
  • Post-translational

    Sumoylation at Lys-14 impairs catalytic activity.
  • Cellular localization

  • Information by UniProt


ab111465 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab111465.
Please use the links above to contact us or submit feedback about this product.

For licensing inquiries, please contact

Sign up