Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 85% Densitometry
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Product nameRecombinant human HIPK4 protein
See all HIPK4 proteins and peptides
The specific activity of ab204158 was determined to be 220 nmol/min/mg.
Purity> 85 % Densitometry.
Expression systemBaculovirus infected Sf9 cells
Protein lengthFull length protein
SequenceMSTIQSETDCYDIIEVLGKGTFGEVAKGWRRSTGEMVAIKILKNDAYRNR IIKNELKLLHCMRGLDPEEAHVIRFLEFFHDALKFYLVFELLEQNLFEFQ KENNFAPLPARHIRTVTLQVLTALARLKELAIIHADLKPENIMLVDQTRC PFRVKVIDFGSASIFSEVRYVKEPYIQSRFYRAPEILLGLPFCEKVDVWS LGCVMAELHLGWPLYPGNNEYDQVRYICETQGLPKPHLLHAACKAHHFFK RNPHPDAANPWQLKSSADYLAETKVRPLERRKYMLKSLDQIETVNGGSVA SRLTFPDREALAEHADLKSMVELIKRMLTWESHERISPSAALRHPFVS MQQLRSAHETTHYYQLSLRSYRLSLQVEGKPPTPVVAAEDGTPYYCLAEE KEAAGMGSVAGSSPFFREEKAPGMQRAIDQLDDLSLQEAGHGLWGETCTN AVSDMMVPLKAAITGHHVPDSGPEPILAFYSSRLAGRHKARKPPAGSKSD SNFSNLIRLSQVSPEDDRPCRGSSWEEGEHLGASAEPLAILQRDEDGPNI DNMTMEAERPDPELFDPSSCPGEWLSEPDCTLESVRGPRAQGLPPRRSHQ HGPPRGATSFLQHVTGHH
Predicted molecular weight85 kDa including tags
Amino acids1 to 616
TagsHis tag N-Terminus
Additional sequence informationNM_144685
Our Abpromise guarantee covers the use of ab204158 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Preservative: 1.02% Imidazole
Constituents: 0.82% Monobasic dihydrogen sodium phosphate, 1.76% Sodium chloride, 0.002% PMSF, 0.004% DTT, 25% Glycerol (glycerin, glycerine)
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- HIPK 4
FunctionProtein kinase that phosphorylates human TP53 at Ser-9, and thus induces TP53 repression of BIRC5 promoter (By similarity). May act as a corepressor of transcription factors.
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. HIPK subfamily.
Contains 1 protein kinase domain.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab204158 has not yet been referenced specifically in any publications.