Recombinant Human Histone H1.0 protein (ab158626)

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SDS-PAGE - Recombinant Human Histone H1.0 protein (ab158626)

    Key features and details

    • Expression system: Wheat germ
    • Tags: GST tag N-Terminus
    • Suitable for: ELISA, WB

    Description

    • Product name

      Recombinant Human Histone H1.0 protein

    • Expression system

      Wheat germ

    • Accession

      P07305

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MTENSTSAPAAKPKRAKASKKSTDHPKYSDMIVAAIQAEKNRAGSSRQSI QKYIKSHYKVGENADSQIKLSIKRLVTTGVLKQTKGVGASGSFRLAKSDE PKKSVAFKKTKKEIKKVATPKKASKPKKAASKAPTKKPKATPVKKAKKKL AATPKKAKKPKTVKAKPVKASKPKKAKPVKPKAKSSAKRAGKKK

      • Predicted molecular weight

        47 kDa including tags

      • Amino acids

        1 to 194

      • Tags

        GST tag N-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab158626 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      ELISA

      Western blot

    • Form

      Liquid

    • Additional notes

       

       

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.31% Glutathione, 0.79% Tris HCl

    General Info

    • Alternative names

      • H1 histone family member 0
      • H1(0)
      • H10
      • H10_HUMAN
      • h1f0
      • H1FV
      • Histone H1''
      • Histone H1(0)
      • Histone H1.0
      • Histone H10
      • Histone H5
      • MGC5241
      • N-terminally processed
      see all

    • Function

      Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division.

    • Sequence similarities

      Belongs to the histone H1/H5 family.
      Contains 1 H15 (linker histone H1/H5 globular) domain.

    • Post-translational
      modifications

      Phosphorylated on Ser-17 in RNA edited version.

    • Cellular localization

      Nucleus. Chromosome. The RNA edited version has been localized to nuclear speckles. During mitosis, it appears in the vicinity of condensed chromosomes.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human Histone H1.0 protein (ab158626)
      SDS-PAGE - Recombinant Human Histone H1.0 protein (ab158626)
      ab158626 on a 12.5% SDS-PAGE stained with Coomassie Blue.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab158626? Please let us know so that we can cite the reference in this datasheet.

    ab158626 has not yet been referenced specifically in any publications.

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