Recombinant Human Histone H2B protein (ab92409)

Description

  • Product name

    Recombinant Human Histone H2B protein
    See all Histone H2B proteins and peptides
  • Purity

    >= 95 % SDS-PAGE.

  • Expression system

    Escherichia coli
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human

Specifications

Our Abpromise guarantee covers the use of ab92409 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    Functional Studies

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

General Info

  • Alternative names

    • GL105
    • H2B GL105
    • H2B histone family member O
    • H2B histone family member S
    • H2B.1
    • H2B.1 B
    • H2B.b
    • H2B.c
    • H2B.d
    • H2B.e
    • H2B.f
    • H2B.j
    • H2B.q
    • H2B/b
    • H2B/c
    • H2B/d
    • H2B/e
    • H2B/f
    • H2B/j
    • H2B/o
    • H2B/q
    • H2BFB
    • H2BFC
    • H2BFD
    • H2BFE
    • H2BFF
    • H2BFJ
    • H2BFO
    • H2BFQ
    • H2BFS
    • H2BGL105
    • H2BQ
    • HIRIP2
    • HIST1H2BB
    • HIST1H2BD
    • HIST1H2BH
    • HIST1H2BL
    • HIST1H2BM
    • HIST1H2BN
    • HIST2H2BE
    • histone cluster 2
    • Histone H2B
    • histone H2B GL105
    • Histone H2B type 1 B
    • Histone H2B type 1 D
    • Histone H2B type 1 H
    • Histone H2B type 1 L
    • Histone H2B type 1 M
    • Histone H2B type 1 N
    • Histone H2B type 2 E
    • Histone H2B.q
    • histone protein
    see all
  • Relevance

    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Subunit structure The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Post-translational modification Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-121 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons. Phosphorylation at Ser-37 (H2BS36ph) by AMPK in response to stress promotes transcription. Phosphorylated on Ser-15 (H2BS14ph) by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination. GlcNAcylation at Ser-113 promotes monoubiquitination of Lys-121. It fluctuates in response to extracellular glucose, and associates with transcribed genes. Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.
  • Cellular localization

    Nuclear

Images

  • Anti-Histone H2B antibody - ChIP Grade (ab1790) at 0.1 µg/ml + Recombinant Human Histone H2B protein (ab92409) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 2 minutes
  • SDS-PAGE Analysis: Lane 1: MW Marker; Lane 2: 1.0 µg Human Histone H2B Protein (ab92409).

  • Western Blot Analysis. Lane 1: MW Marker; Lane 2: 100 ng Human Histone H2B Protein (ab92409) probed with Histone H2B pAb.

References

ab92409 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

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