Recombinant Human Histone H4 protein (ab198115)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      SGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLI YEETRGVLKVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYGF GG
    • Molecular weight
      12 kDa including tags
    • Amino acids
      2 to 103
    • Tags
      His tag N-Terminus
    • Additional sequence information
      GenBank Accession No. NM_003548

Specifications

Our Abpromise guarantee covers the use of ab198115 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity
    >= 93 % SDS-PAGE.

  • Form
    Liquid
  • Additional notes

    ab198115 is useful as a substrate for histone methyltransferase and acetyltransferase assays. Ideal for screening small molecular inhibitors of histone modifying enzymes for drug discovery and HTS applications.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 7.4
    Constituents: 0.64% Sodium chloride, 0.02% Potassium chloride, 0.05% DTT, 20% Glycerol, 79% PBS

General Info

  • Alternative names
    • dJ160A22.1
    • dJ160A22.2
    • dJ221C16.1
    • dJ221C16.9
    • FO108
    • H4
    • H4.k
    • H4/a
    • H4/b
    • H4/c
    • H4/d
    • H4/e
    • H4/g
    • H4/h
    • H4/I
    • H4/j
    • H4/k
    • H4/m
    • H4/n
    • H4/p
    • H4_HUMAN
    • H4F2
    • H4F2iii
    • H4F2iv
    • H4FA
    • H4FB
    • H4FC
    • H4FD
    • H4FE
    • H4FG
    • H4FH
    • H4FI
    • H4FJ
    • H4FK
    • H4FM
    • H4FN
    • H4M
    • HIST1H4A
    • HIST1H4B
    • HIST1H4C
    • HIST1H4D
    • HIST1H4E
    • HIST1H4F
    • HIST1H4H
    • HIST1H4I
    • HIST1H4J
    • HIST1H4K
    • HIST1H4L
    • HIST2H4
    • HIST2H4A
    • Hist4h4
    • Histone 1 H4a
    • Histone 1 H4b
    • Histone 1 H4c
    • Histone 1 H4d
    • Histone 1 H4e
    • Histone 1 H4f
    • Histone 1 H4h
    • Histone 1 H4i
    • Histone 1 H4j
    • Histone 1 H4k
    • Histone 1 H4l
    • Histone 2 H4a
    • histone 4 H4
    • Histone H4
    • MGC24116
    see all
  • Function
    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • Sequence similarities
    Belongs to the histone H4 family.
  • Post-translational
    modifications
    Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
    Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
    Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
    Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
    Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
    Sumoylated, which is associated with transcriptional repression.
  • Cellular localization
    Nucleus. Chromosome.
  • Information by UniProt

Images

  • 4-20% SDS-PAGE analysis of ab198115.

    Lane 1: 2 µg ab198115
    Lane 2: Protein marker

    Stained with Coomassie Blue

References

ab198115 has not yet been referenced specifically in any publications.

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