Recombinant Human HIV-1 gp120 (CN54) protein (ab174070)

Our Abpromise guarantee covers the use of ab174070 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Stability and Storage

Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Information available upon request.
Reconstitution

It is recommended to reconstitute the lyophilized product in 100 µl sterile deionized water to a final concentration of 1 mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage. After reconstitution store under sterile conditions for 1 month at 4°C - 8°C or 3 months at -20°C to -80°C. Aliquot to avoid repeated freeze-thaw cycles.

Alternative names
- Envelope glycoprotein gp160
Relevance

The envelope glyprotein gp160 precursor down-modulates cell surface CD4 antigen by interacting with it in the endoplasmic reticulum and blocking its transport to the cell surface By similarity. The gp120-gp41 heterodimer allows rapid transcytosis of the virus through CD4 negative cells such as simple epithelial monolayers of the intestinal, rectal and endocervical epithelial barriers. Both gp120 and gp41 specifically recognize glycosphingolipids galactosyl-ceramide (GalCer) or 3' sulfo-galactosyl-ceramide (GalS) present in the lipid rafts structures of epithelial cells. Binding to these alternative receptors allows the rapid transcytosis of the virus through the epithelial cells. This transcytotic vesicle-mediated transport of virions from the apical side to the basolateral side of the epithelial cells does not involve infection of the cells themselves.

SDS-PAGE - Recombinant Human HIV-1 gp120 (CN54) protein (ab174070)

SDS-PAGE analysis of reduced ab174070 stained overnight with Coomassie Blue.
DTT-reduced Protein migrates as 65-110 kDa due to glycosylation.

ab174070 has not yet been referenced specifically in any publications.

Publishing research using ab174070? Please let us know so that we can cite the reference in this datasheet.

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