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I'm writing regarding your product: HMGB1 protein (His tag) (ab81876).
In the datasheet on your website it is stated that "GSA: 1 unit equals 1 nanogram of purified protein. 1 unit is sufficient for a gel mobility shift assay in a 20 µl reaction to super-shift TBP-DNA complex".
I was wondering whether you have any more information on this, such as a relevant protocol?
Asked on Dec 06 2012
I am sorry for the delay in getting back to you in regards to the HMGB1 protein (His tag) (ab81876).
I now have further information to share. The protein has been used in gel mobility shift assay as described on the datasheet of ab81876. the description of 1 unit is a general guideline. A range of 0.1 ng-5 ng has been used for gel mobility shift experiments dependent on the proteins. For HMG1 you may need to increase this to 2-5 ng (Unit).
The protocol which was followed has been described in the reference attached to this email.
I hope this information has been of help. If I can be of any further assistance, please do not hesitate to let me know.
Answered on Dec 06 2012