Description

  • Product name

    Recombinant Human HSF1 (phospho ) protein
    See all HSF1 proteins and peptides
  • Purity

    > 90 % SDS-PAGE.
    Purity =90% (~60% full-length with trace upstream kinase).
  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Predicted molecular weight

      70 kDa including tags
    • Amino acids

      1 to 529
    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab115508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Western blot

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Constituents: 99% Tris buffered saline, 5% Glycerol

General Info

  • Alternative names

    • Heat shock factor 1
    • Heat shock factor protein 1
    • Heat shock transcription factor 1
    • HSF 1
    • hsf1
    • HSF1_HUMAN
    • HSTF 1
    • HSTF1
    see all
  • Function

    DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
  • Sequence similarities

    Belongs to the HSF family.
  • Domain

    the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
  • Post-translational
    modifications

    Phosphorylated on multiple serine residues, a subset of which are involved in stress-related regulation of transcription activation. Constitutive phosphorylation represses transcriptional activity at normal temperatures. Levels increase on specific residues heat-shock and enhance HSF1 transactivation activity. Phosphorylation on Ser-307 derepresses activation on heat-stress and in combination with Ser-303 phosphorylation appears to be involved in recovery after heat-stress. Phosphorylated on Ser-230 by CAMK2, in vitro. Cadmium also enhances phosphorylation at this site. Phosphorylation on Ser-303 is a prerequisite for HSF1 sumoylation. Phosphorylation on Ser-121 inhibits transactivation and promotes HSP90 binding. Phosphorylation on Thr-142 also mediates transcriptional activity induced by heat. Phosphorylation on Ser-326 plays an important role in heat activation of HSF1 transcriptional activity.
    Sumoylated with SUMO1 and SUMO2 on heat-shock. Heat-inducible sumoylation occurs after 15 min of heat-shock, after which levels decrease and at 4 hours, levels return to control levels. Sumoylation has no effect on HSE binding nor on transcriptional activity. Phosphorylation on Ser-303 is a prerequisite for sumoylation.
  • Cellular localization

    Cytoplasm. Nucleus. Cytoplasmic during normal growth. On activation, translocates to nuclear stress granules. Colocalizes with SUMO1 in nuclear stress granules.
  • Information by UniProt

Images

  • SDS-PAGE analysis:
    Lane 1: MWM,
    Lane 2: 2µg HSF1,
    Lane 3: 2µg ab115508 (HSF1 - phospho)
  • Western Blot analysis:
    Lane 1: MW marker
    Lane 2: HSF1
    Lane 3: HSF1 (phospho) probed with HSF1 pAb
  • Western Blot analysis:
    Lane 1: MW marker
    Lane 2: HSF1
    Lane 3: HSF1 (phospho) probed with HSF1 (pSer326)

References

ab115508 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Question
Answer

Thank you for your call last week and for your patience while I have been in touch with the lab regarding your enquiry.

I have heard back from the lab, and they have confirmed that ab117515 is phosphorylated in vitro to produce ab115508.

I hope this information will be useful, but please let me know if you have further questions or if there is anything else that we can do for you.

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