Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, WB
Product nameRecombinant Human HSF1 (phospho ) protein
See all HSF1 proteins and peptides
Purity> 90 % SDS-PAGE.
Purity =90% (~60% full-length with trace upstream kinase).
Expression systemEscherichia coli
Protein lengthFull length protein
Predicted molecular weight70 kDa including tags
Amino acids1 to 529
TagsHis tag N-Terminus
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-HSF1 antibody (ab2923)
- Anti-HSF1 (phospho S303) antibody (ab47369)
- Anti-6X His tag® antibody [4D11] (ab5000)
- Anti-HSF1 antibody [10H8] (ab61382)
- Anti-HSF1 (phospho S326) antibody [EP1713Y] (ab76076)
- Anti-HSF1 (phospho S320) antibody [EPR1712] (ab76183)
Our Abpromise guarantee covers the use of ab115508 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: Tris buffered saline, 5% Glycerol
- Heat shock factor 1
- Heat shock factor protein 1
- Heat shock transcription factor 1
FunctionDNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
Sequence similaritiesBelongs to the HSF family.
Domainthe 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
modificationsPhosphorylated on multiple serine residues, a subset of which are involved in stress-related regulation of transcription activation. Constitutive phosphorylation represses transcriptional activity at normal temperatures. Levels increase on specific residues heat-shock and enhance HSF1 transactivation activity. Phosphorylation on Ser-307 derepresses activation on heat-stress and in combination with Ser-303 phosphorylation appears to be involved in recovery after heat-stress. Phosphorylated on Ser-230 by CAMK2, in vitro. Cadmium also enhances phosphorylation at this site. Phosphorylation on Ser-303 is a prerequisite for HSF1 sumoylation. Phosphorylation on Ser-121 inhibits transactivation and promotes HSP90 binding. Phosphorylation on Thr-142 also mediates transcriptional activity induced by heat. Phosphorylation on Ser-326 plays an important role in heat activation of HSF1 transcriptional activity.
Sumoylated with SUMO1 and SUMO2 on heat-shock. Heat-inducible sumoylation occurs after 15 min of heat-shock, after which levels decrease and at 4 hours, levels return to control levels. Sumoylation has no effect on HSE binding nor on transcriptional activity. Phosphorylation on Ser-303 is a prerequisite for sumoylation.
Cellular localizationCytoplasm. Nucleus. Cytoplasmic during normal growth. On activation, translocates to nuclear stress granules. Colocalizes with SUMO1 in nuclear stress granules.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab115508 has not yet been referenced specifically in any publications.