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    recombinant-human-hsf1-protein-ab78795.pdf

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Tags & Cell Markers Subcellular Markers Nucleus Other Nuclear Bodies
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Recombinant Human HSF1 protein (ab78795)

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SDS-PAGE - Recombinant Human HSF1 protein (ab78795)
  • Western blot - Recombinant Human HSF1 protein (ab78795)

Key features and details

  • Expression system: Escherichia coli
  • Suitable for: SDS-PAGE, WB

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ELISA
Product image
Human Heat Shock Factor Protein 1 ELISA kit (ab222879)

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Description

  • Product name

    Recombinant Human HSF1 protein
    See all HSF1 proteins and peptides
  • Expression system

    Escherichia coli
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MGSSHHHHHH SSGLVPRGSH MDLPVGPGAA GPSNVPAFLT KLWTLVSDPD TDALICWSPS GNSFHVFDQG QFAKEVLPKY FKHNNMASFV RQLNMYGFRK VVHIEQGGLV KPERDDTEFQ HPCFLRGQEQ LLENIKRKVT SVSTLKSEDI KIRQDSVTKL LTDVQLMKGK QECMDSKLLA MKHENEALWR EVASLRQKHA QQQKVVNKLI QFLISLVQSN RILGVKRKIP LMLNDSGSAH SMPKYSRQFS LEHVHGSGPY SAPSPAYSSS SLYAPDAVAS SGPIISDITE LAPASPMASP GGSIDERPLS SSPLVRVKEE PPSPPQSPRV EEASPGRPSS VDTLLSPTAL IDSILRESEP APASVTALTD ARGHTDTEGR PPSPPPTSTP EKCLSVACLD KNELSDHLDA MDSNLDNLQT MLSSHGFSVD TSALLDLFSP SVTVPDMSLP DLDSSLASIQ ELLSPQEPPR PPEAENSSPD SGKQLVHYTA QPLFLLDPGS VDTGSNDLPV LFELGEGSYF SEGDGFAEDP TISLLTGSEP PKAKDPTVS

Associated products

  • Related Products

    • Anti-HSF1 antibody (ab105086)
    • Anti-6X His tag® antibody [HIS.H8] (ab18184)
    • Anti-HSF1 antibody (ab2923)
    • Anti-HSF1 (phospho S303) antibody (ab47369)
    • Anti-6X His tag® antibody [4D11] (ab5000)
    • Anti-HSF1 antibody [EP1710Y] - ChIP Grade (ab52757)
    • Anti-HSF1 antibody [10H8] (ab61382)
    • Anti-HSF1 (phospho S326) antibody [EP1713Y] (ab76076)
    • Anti-HSF1 (phospho S320) antibody [EPR1712] (ab76183)

Specifications

Our Abpromise guarantee covers the use of ab78795 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Western blot

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.0154% DTT, 0.242% Tris, 0.29% Sodium chloride

General Info

  • Alternative names

    • Heat shock factor 1
    • Heat shock factor protein 1
    • Heat shock transcription factor 1
    • HSF 1
    • hsf1
    • HSF1_HUMAN
    • HSTF 1
    • HSTF1
    see all
  • Function

    DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
  • Sequence similarities

    Belongs to the HSF family.
  • Domain

    the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
  • Post-translational
    modifications

    Phosphorylated on multiple serine residues, a subset of which are involved in stress-related regulation of transcription activation. Constitutive phosphorylation represses transcriptional activity at normal temperatures. Levels increase on specific residues heat-shock and enhance HSF1 transactivation activity. Phosphorylation on Ser-307 derepresses activation on heat-stress and in combination with Ser-303 phosphorylation appears to be involved in recovery after heat-stress. Phosphorylated on Ser-230 by CAMK2, in vitro. Cadmium also enhances phosphorylation at this site. Phosphorylation on Ser-303 is a prerequisite for HSF1 sumoylation. Phosphorylation on Ser-121 inhibits transactivation and promotes HSP90 binding. Phosphorylation on Thr-142 also mediates transcriptional activity induced by heat. Phosphorylation on Ser-326 plays an important role in heat activation of HSF1 transcriptional activity.
    Sumoylated with SUMO1 and SUMO2 on heat-shock. Heat-inducible sumoylation occurs after 15 min of heat-shock, after which levels decrease and at 4 hours, levels return to control levels. Sumoylation has no effect on HSE binding nor on transcriptional activity. Phosphorylation on Ser-303 is a prerequisite for sumoylation.
  • Cellular localization

    Cytoplasm. Nucleus. Cytoplasmic during normal growth. On activation, translocates to nuclear stress granules. Colocalizes with SUMO1 in nuclear stress granules.
  • Target information above from: UniProt accession Q00613 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Images

  • SDS-PAGE - Recombinant Human HSF1 protein (ab78795)
    SDS-PAGE - Recombinant Human HSF1 protein (ab78795)
    15% SDS-PAGE showing ab78795 at approximately 59kDa (3µg).
  • Western blot - Recombinant Human HSF1 protein (ab78795)
    Western blot - Recombinant Human HSF1 protein (ab78795)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab78795? Please let us know so that we can cite the reference in this datasheet.

    ab78795 has not yet been referenced specifically in any publications.

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