Recombinant Human Hsp60 protein (ab113177)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Endotoxin level: < 50.000 Eu/mg
- Suitable for: WB, Functional Studies, SDS-PAGE
Description
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Product name
Recombinant Human Hsp60 protein
See all Hsp60 proteins and peptides -
Purity
> 90 % SDS-PAGE.
ab113177 was purified by multi-step chromatography. -
Endotoxin level
< 50.000 Eu/mg -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
61 kDa -
Amino acids
1 to 573
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab113177 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
Functional Studies
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituents: 99% PBS, Phosphate Buffer
General Info
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Alternative names
- 60 kDa chaperonin
- 60 kDa heat shock protein, mitochondrial
- CH60_HUMAN
see all -
Function
Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. -
Involvement in disease
Defects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13) [MIM:605280]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4) [MIM:612233]; also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life. -
Sequence similarities
Belongs to the chaperonin (HSP60) family. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
Images
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SDS-PAGE analysis of ab113177: Lane 1: MW marker, Lane 2: 0.5ug, Lane 3: 1ug, Lane 4: 2.5ug, Lane 5: 5ug, Lane 6: 10ug.
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All lanes : a monoclonal anti Hsp60 protein
Lane 1 : molecualr weight marker
Lane 2 :Recombinant Human Hsp60 protein (ab113177) at 0.1 µg
Lane 3 :Recombinant Human Hsp60 protein (ab113177) at 0.05 µg
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab113177 has been referenced in 1 publication.
- Shi L et al. Anti-heat shock protein autoantibody profiling in breast cancer using customized protein microarray. Anal Bioanal Chem 408:1497-506 (2016). PubMed: 26715250