Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Endotoxin level: < 50.000 Eu/mg
- Suitable for: WB, Functional Studies, SDS-PAGE
Product nameRecombinant Human Hsp60 protein
See all Hsp60 proteins and peptides
Purity> 90 % SDS-PAGE.
ab113177 was purified by multi-step chromatography.
Endotoxin level< 50.000 Eu/mg
Expression systemEscherichia coli
Protein lengthFull length protein
Predicted molecular weight61 kDa
Amino acids1 to 573
Our Abpromise guarantee covers the use of ab113177 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituents: 99% PBS, Phosphate Buffer
- 60 kDa chaperonin
- 60 kDa heat shock protein, mitochondrial
FunctionImplicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.
Involvement in diseaseDefects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13) [MIM:605280]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4) [MIM:612233]; also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life.
Sequence similaritiesBelongs to the chaperonin (HSP60) family.
Cellular localizationMitochondrion matrix.
- Information by UniProt
SDS-PAGE analysis of ab113177: Lane 1: MW marker, Lane 2: 0.5ug, Lane 3: 1ug, Lane 4: 2.5ug, Lane 5: 5ug, Lane 6: 10ug.
All lanes : a monoclonal anti Hsp60 protein
Lane 1 : molecualr weight marker
Lane 2 :
Recombinant Human Hsp60 protein (ab113177) at 0.1 µg
Lane 3 :
Recombinant Human Hsp60 protein (ab113177) at 0.05 µg
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab113177 has not yet been referenced specifically in any publications.