Product nameRecombinant human Hsp70 protein
See all Hsp70 proteins and peptides
Biological activityab78427 has ATPase activity at the time of manufacture of 3.3µM phosphate liberated/hr/µg protein in a 200µl reaction at 37°C (pH7.5) in the presence of 20ul of 1mM ATP using a Malachite Green assay.
Purity> 90 % SDS-PAGE.
ab78427 is affinity purified.
Expression systemEscherichia coli
Protein lengthFull length protein
SequenceMAKAAAIGID LGTTYSCVGV FQHGKVEIIA NDQGNRTTPS YVAFTDTERL IGDAAKNQVA LNPQNTVFDA KRLIGRKFGD PVVQSDMKHW PFQVINDGDK PKVQVSYKGE TKAFYPEEIS SMVLTKMKEI AEAYLGYPVT NAVITVPAYF NDSQRQATKD AGVIAGLNVL RIINEPTAAA IAYGLDRTGK GERNVLIFDL GGGTFDVSIL TIDDGIFEVK ATAGDTHLGG EDFDNRLVNH FVEEFKRKHK KDISQNKRAV RRLRTACERA KRTLSSSTQA SLEIDSLFEG IDFYTSITRA RFEELCSDLF RSTLEPVEKA LRDAKLDKAQ IHDLVLVGGS TRIPKVQKLL QDFFNGRDLN KSINPDEAVA YGAAVQAAIL MGDKSENVQD LLLLDVAPLS LGLETAGGVM TALIKRNSTI PTKQTQIFTT YSDNQPGVLI QVYEGERAMT KDNNLLGRFE LSGIPPAPRG VPQIEVTFDI DANGILNVTA TDKSTGKANK ITITNDKGRL SKEEIERMVQ EAEKYKAEDE VQRERVSAKN ALESYAFNMK SAVEDEGLKG KISEADKKKV LDKCQEVISW LDANTLAEKD EFEHKRKELE QVCNPIISGL YQGAGGPGPG GFGAQGPKGG SGSGPTIEEV D
TagsHis tag N-Terminus
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-Hsp70 antibody [5A5] (ab2787)
- Anti-Hsp70 antibody (ab31010)
- Anti-Hsp70 antibody [EP1007Y] (ab45133)
- Anti-Hsp70 antibody [C92F3A-5] (ab47455)
- Anti-6X His tag® antibody [4D11] (ab5000)
- Anti-Hsp70 antibody [3A3] (ab5439)
- Anti-Hsp70 antibody [2A4] (ab5442)
Our Abpromise guarantee covers the use of ab78427 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 1.36% Imidazole
Constituents: 0.87% Sodium chloride, 10% Glycerol, 0.328% Sodium phosphate
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- DnaK type molecular chaperone HSP70 1
- Epididymis secretory protein Li 103
RelevanceFunction: In cooperation with other chaperones, the Hsp70 family stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell. Tissue specificity: HSPA1B is testis-specific.
Cellular localizationCytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab78427 has not yet been referenced specifically in any publications.