Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, WB, Functional Studies
Product nameRecombinant human Hsp70 protein
See all Hsp70 proteins and peptides
Purity> 90 % SDS-PAGE.
Purified by Multi-step chromatography. > 90% pure as determined by SDS-PAGE and Western Blot analysis.
Expression systemEscherichia coli
Protein lengthFull length protein
Our Abpromise guarantee covers the use of ab92415 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Additional notesEndotoxin level: < 50 EU/mg as determined by Limulus Amebocyte Lysate (LAL) gel clot assay.ATPase assay: positive
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituent: 2.68% PBS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- DnaK type molecular chaperone HSP70 1
- Epididymis secretory protein Li 103
RelevanceFunction: In cooperation with other chaperones, the Hsp70 family stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell. Tissue specificity: HSPA1B is testis-specific.
Cellular localizationCytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab92415 has been referenced in 1 publication.
- Tseng CC et al. GRP78 regulates CD44v membrane homeostasis and cell spreading in tamoxifen-resistant breast cancer. Life Sci Alliance 2:N/A (2019). PubMed: 31416894