Recombinant human Hsp70 protein (Active) (ab78434)
Key features and details
- Expression system: Baculovirus infected BTI-TN-5B1-4 cells
- Purity: > 90% SDS-PAGE
- Endotoxin level: < 0.700 Eu/ml
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: WB, ELISA, Functional Studies, Competitive Binding Assays, SDS-PAGE
Description
-
Product name
Recombinant human Hsp70 protein (Active)
See all Hsp70 proteins and peptides -
Biological activity
ab78434 tested positive for ATPase activity using a Malachite Green assay.
-
Purity
> 90 % SDS-PAGE.
ab78434 is affinity purified. -
Endotoxin level
< 0.700 Eu/ml -
Expression system
Baculovirus infected BTI-TN-5B1-4 cells -
Accession
-
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Sequence
MAKAAAIGID LGTTYSCVGV FQHGKVEIIA NDQGNRTTPS YVAFTDTERL IGDAAKNQVA LNPQNTVFDA KRLIGRKFGD PVVQSDMKHW PFQVINDGDK PKVQVSYKGE TKAFYPEEIS SMVLTKMKEI AEAYLGYPVT NAVITVPAYF NDSQRQATKD AGVIAGLNVL RIINEPTAAA IAYGLDRTGK GERNVLIFDL GGGTFDVSIL TIDDGIFEVK ATAGDTHLGG EDFDNRLVNH FVEEFKRKHK KDISQNKRAV RRLRTACERA KRTLSSSTQA SLEIDSLFEG IDFYTSITRA RFEELCSDLF RSTLEPVEKA LRDAKLDKAQ IHDLVLVGGS TRIPKVQKLL QDFFNGRDLN KSINPDEAVA YGAAVQAAIL MGDKSENVQD LLLLDVAPLS LGLETAGGVM TALIKRNSTI PTKQTQIFTT YSDNQPGVLI QVYEGERAMT KDNNLLGRFE LSGIPPAPRG VPQIEVTFDI DANGILNVTA TDKSTGKANK ITITNDKGRL SKEEIERMVQ EAEKYKAEDE VQRERVSAKN ALESYAFNMK SAVEDEGLKG KISEADKKKV LDKCQEVISW LDANTLAEKD EFEHKRKELE QVCNPIISGL YQGAGGPGPG GFGAQGPKGG SGSGPTIEEV D -
Predicted molecular weight
70 kDa -
Amino acids
1 to 641 -
Tags
His tag N-Terminus -
Additional sequence information
(NM_005345) (Gene ID 3303) (P0DMV9)
-
Associated products
-
Related Products
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-Hsp70 antibody [5A5] (ab2787)
- Anti-Hsp70 antibody (ab31010)
- Anti-Hsp70 antibody [EP1007Y] (ab45133)
- Anti-Hsp70 antibody [C92F3A-5] (ab47455)
- Anti-6X His tag® antibody [4D11] (ab5000)
- Anti-Hsp70 antibody [3A3] (ab5439)
- Anti-Hsp70 antibody [2A4] (ab5442)
Specifications
Our Abpromise guarantee covers the use of ab78434 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
Applications
Western blot
ELISA
Functional Studies
Competitive Binding Assays
SDS-PAGE
-
Form
Liquid -
Concentration information loading...
Preparation and Storage
-
Stability and Storage
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Constituents: 0.87% Sodium chloride, 0.24% Tris, 10% Glycerol
This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
-
Alternative names
- DnaK type molecular chaperone HSP70 1
- Epididymis secretory protein Li 103
- FLJ54303
see all -
Relevance
Function: In cooperation with other chaperones, the Hsp70 family stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell. Tissue specificity: HSPA1B is testis-specific. -
Cellular localization
Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab78434 has not yet been referenced specifically in any publications.