Description

  • Product name

    Recombinant human Hsp70 protein (Active)
    See all Hsp70 proteins and peptides
  • Biological activity

    ab78434 tested positive for ATPase activity using a Malachite Green assay.

  • Purity

    > 90 % SDS-PAGE.
    ab78434 is affinity purified.
  • Endotoxin level

    < 0.700 Eu/ml
  • Expression system

    Baculovirus infected BTI-TN-5B1-4 cells
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MAKAAAIGID LGTTYSCVGV FQHGKVEIIA NDQGNRTTPS YVAFTDTERL IGDAAKNQVA LNPQNTVFDA KRLIGRKFGD PVVQSDMKHW PFQVINDGDK PKVQVSYKGE TKAFYPEEIS SMVLTKMKEI AEAYLGYPVT NAVITVPAYF NDSQRQATKD AGVIAGLNVL RIINEPTAAA IAYGLDRTGK GERNVLIFDL GGGTFDVSIL TIDDGIFEVK ATAGDTHLGG EDFDNRLVNH FVEEFKRKHK KDISQNKRAV RRLRTACERA KRTLSSSTQA SLEIDSLFEG IDFYTSITRA RFEELCSDLF RSTLEPVEKA LRDAKLDKAQ IHDLVLVGGS TRIPKVQKLL QDFFNGRDLN KSINPDEAVA YGAAVQAAIL MGDKSENVQD LLLLDVAPLS LGLETAGGVM TALIKRNSTI PTKQTQIFTT YSDNQPGVLI QVYEGERAMT KDNNLLGRFE LSGIPPAPRG VPQIEVTFDI DANGILNVTA TDKSTGKANK ITITNDKGRL SKEEIERMVQ EAEKYKAEDE VQRERVSAKN ALESYAFNMK SAVEDEGLKG KISEADKKKV LDKCQEVISW LDANTLAEKD EFEHKRKELE QVCNPIISGL YQGAGGPGPG GFGAQGPKGG SGSGPTIEEV D
    • Predicted molecular weight

      70 kDa
    • Amino acids

      1 to 641
    • Additional sequence information

      (NM_005345) (Gene ID 3303) (P0DMV9)

Specifications

Our Abpromise guarantee covers the use of ab78434 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    ELISA

    Functional Studies

    competitive binding assays

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

    pH: 8.00
    Constituents: 0.87% Sodium chloride, 0.24% Tris, 10% Glycerol

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • DnaK type molecular chaperone HSP70 1
    • Epididymis secretory protein Li 103
    • FLJ54303
    • FLJ54370
    • FLJ54392
    • FLJ54408
    • FLJ75127
    • Heat shock 70 kDa protein 1
    • Heat shock 70 kDa protein 1/2
    • Heat shock 70 kDa protein 1A
    • Heat shock 70 kDa protein 1A/1B
    • Heat shock 70kDa protein 1B
    • Heat shock induced protein
    • HEL S 103
    • HSP70 1
    • HSP70 1A
    • HSP70 1B
    • HSP70 2
    • HSP70-1
    • HSP70-1/HSP70-2
    • HSP70-1A
    • HSP70-2
    • HSP70.1
    • HSP70.1/HSP70.2
    • HSP71_HUMAN
    • HSP72
    • HSPA1
    • HSPA1A
    • HSPA1B
    see all
  • Relevance

    Function: In cooperation with other chaperones, the Hsp70 family stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell. Tissue specificity: HSPA1B is testis-specific.
  • Cellular localization

    Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Images

References

ab78434 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Question
Answer

This protein has a His-tag

Read More

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