Description

  • Product name

    Recombinant human Hsp90 alpha protein (Active)
    See all Hsp90 alpha proteins and peptides
  • Biological activity

    102 pmol/min/µg

    Assay conditions : Buffer contains 0.1M HEPES, pH 7.4, 0.25M MgCl2, 0.25M KCl, 0.1M Na2MoO4, and 0.05% Triton X. Incubated for 150 minutes at room temperature.

    Fluorescence polarization measured at exc470/em528.

  • Purity

    >= 90 % SDS-PAGE.

  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MPEETQTQDQ PMEEEEVETF AFQAEIAQLM SLIINTFYSN KEIFLRELIS NSSDALDKIR YESLTDPSKL DSGKELHINL IPNKQDRTLT IVDTGIGMTK ADLINNLGTI AKSGTKAFME ALQAGADISM IGQFGVGFYS AYLVAEKVTV ITKHNDDEQY AWESSAGGSF TVRTDTGEPM GRGTKVILHL KEDQTEYLEE RRIKEIVKKH SQFIGYPITL FVEKERDKEV SDDEAEEKED KEEEKEKEEK ESEDKPEIED VGSDEEEEKK DGDKKKKKKI KEKYIDQEEL NKTKPIWTRN PDDITNEEYG EFYKSLTNDW EDHLAVKHFS VEGQLEFRAL LFVPRRAPFD LFENRKKKNN IKLYVRRVFI MDNCEELIPE YLNFIRGVVD SEDLPLNISR EMLQQSKILK VIRKNLVKKC LELFTELAED KENYKKFYEQ FSKNIKLGIH EDSQNRKKLS ELLRYYTSAS GDEMVSLKDY CTRMKENQKH IYYITGETKD QVANSAFVER LRKHGLEVIY MIEPIDEYCV QQLKEFEGKT LVSVTKEGLE LPEDEEEKKK QEEKKTKFEN LCKIMKDILE KKVEKVVVSN RLVTSPCCIV TSTYGWTANM ERIMKAQALR DNSTMGYMAA KKHLEINPDH SIIETLRQKA EADKNDKSVK DLVILLYETA LLSSGFSLED PQTHANRIYR MIKLGLGIDE DDPTADDTSA AVTEEMPPLE GDDDTSRMEE VDHHHHHH
    • Predicted molecular weight

      86 kDa including tags
    • Amino acids

      1 to 732
    • Tags

      His tag C-Terminus
    • Additional sequence information

      (NM_005348)

Specifications

Our Abpromise guarantee covers the use of ab80369 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Upon reconsitution add a carrier protein (0.1% BSA). Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Preservative: 1.7% Imidazole
    Constituents: 0.462% DTT, 0.632% Tris HCl, 10% Glycerol, 0.638% Sodium chloride, 0.0165% Potassium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • EL52
    • epididymis luminal secretory protein 52
    • Heat shock 86 kDa
    • heat shock 90kD protein 1, alpha
    • Heat shock 90kD protein 1, alpha like 4
    • heat shock 90kD protein, alpha-like 4
    • Heat shock 90kDa protein 1 alpha
    • Heat shock protein 90kDa alpha (cytosolic) class A member 1
    • Heat shock protein HSP 90-alpha
    • HS90A_HUMAN
    • HSP 86
    • HSP86
    • Hsp89
    • HSP89A
    • Hsp90
    • HSP90A
    • HSP90AA1
    • HSP90ALPHA
    • HSP90N
    • HSPC1
    • HSPCA
    • HSPCAL1
    • HSPCAL4
    • HSPN
    • LAP 2
    • LAP2
    • lipopolysaccharide-associated protein 2
    • LPS-associated protein 2
    • Renal carcinoma antigen NY-REN-38
    see all
  • Function

    Molecular chaperone. Has ATPase activity.
  • Sequence similarities

    Belongs to the heat shock protein 90 family.
  • Post-translational
    modifications

    ISGylated.
  • Cellular localization

    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

Images

  • Specific activity of ab80369.

  • 2 ug of ab80369 on 4-20% SDS-PAGE, stained with Coomassie.

References

ab80369 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Question
Answer

There are 2 main reasons provided by laboratory for loss of activity of this protein;

1. The 4C temperature is not ideal for this protein. It is a very sensitive to heat and should be stored at -80C. One should expect loss in activity when stored it at 4C which is why we do not recommend keeping it at 4C more than couple of hours.

2. Freeze thaw steps : Additional freeze thaw step after receiving the protein would have damaged the protein. We recommend aliquoting Hsp90α into single use aliquots and storing them at -80˚C immediately

FITC geldanamycin should be also freshly diluted from stock each time, and cannot be kept on ice for more than several hours.

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Answer

Thank you for contacting us.

The final concentration of FITC-labeled geldanamycin in the reaction mixture of the binding activity assay was 5 nM.

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Answer

Thank you for contacting us.

The endotoxins has not been removed from this product and the amount also has not been determined yet so I am sorry I may not be helpful with this particular inquiry. I apologize for any inconvenience this may have caused.

I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Question
Answer

Thank you for contacting us. The His tags for all four proteins you asked about are all 6X tags. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.  

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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