Recombinant human Hsp90 alpha protein (Carboxyterminal end) (ab196407)

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Key features and details

  • Expression system: Escherichia coli
  • Purity: > 90% SDS-PAGE
  • Active: Yes
  • Tags: His tag N-Terminus, proprietary tag N-Terminus
  • Suitable for: Functional Studies, SDS-PAGE

Description

  • Product name

    Recombinant human Hsp90 alpha protein (Carboxyterminal end)
    See all Hsp90 alpha proteins and peptides

  • Purity

    > 90 % SDS-PAGE.

  • Expression system

    Escherichia coli

  • Accession

    P07900

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MHHHHHHGGGLNDIFEAQKIEWHEEFEGKTLVSVTKEGLELPEDEEEKKK QEEKKTKFENLCKIMKDILEKKVEKVVVSNRLVTSPCCIVTSTYGWTANM ERIMKAQALRDNSTMGYMAAKKHLEINPDHSIIETLRQKAEADKNDKSVK DLVILLYETALLSSGFSLEDPQTHANRIYRMIKLGLGIDEDDPTADDTSA AVTEEMPPLEGDDDTSRMEEVD

    • Predicted molecular weight

      25 kDa including tags

    • Amino acids

      535 to 732

    • Tags

      His tag N-Terminus , proprietary tag N-Terminus

    • Additional sequence information

      NM_005348. Expressed in an E. coli expression system and enzymatically Biotin-labeled using Avi-tag ™ technology.

Specifications

Our Abpromise guarantee covers the use of ab196407 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.64% Tris HCl, 0.64% Sodium chloride, 0.017% Potassium chloride, 0.04% Tween, 20% Glycerol

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • EL52
    • epididymis luminal secretory protein 52
    • Heat shock 86 kDa
    • heat shock 90kD protein 1, alpha
    • Heat shock 90kD protein 1, alpha like 4
    • heat shock 90kD protein, alpha-like 4
    • Heat shock 90kDa protein 1 alpha
    • Heat shock protein 90kDa alpha (cytosolic) class A member 1
    • Heat shock protein HSP 90-alpha
    • HS90A_HUMAN
    • HSP 86
    • HSP86
    • Hsp89
    • HSP89A
    • Hsp90
    • HSP90A
    • HSP90AA1
    • HSP90ALPHA
    • HSP90N
    • HSPC1
    • HSPCA
    • HSPCAL1
    • HSPCAL4
    • HSPN
    • LAP 2
    • LAP2
    • lipopolysaccharide-associated protein 2
    • LPS-associated protein 2
    • Renal carcinoma antigen NY-REN-38
    see all

  • Function

    Molecular chaperone. Has ATPase activity.

  • Sequence similarities

    Belongs to the heat shock protein 90 family.

  • Post-translational
    modifications

    ISGylated.

  • Cellular localization

    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

Images

  • Functional Studies - Recombinant human Hsp90 alpha protein (Carboxyterminal end) (ab196407)
    Functional Studies - Recombinant human Hsp90 alpha protein (Carboxyterminal end) (ab196407)

    Hsp90 alpha protein (Carboxyterminal end):PPID binding activity measured using Hsp90 alpha protein (Carboxyterminal end) a Inhibitor Screening Assay Kit,

  • SDS-PAGE - Recombinant human Hsp90 alpha protein (Carboxyterminal end) (ab196407)
    SDS-PAGE - Recombinant human Hsp90 alpha protein (Carboxyterminal end) (ab196407)

    4-20% SDS-PAGE analysis of ab196407 at 2 µg stained with Coomassie Blue.

  • Western blot - Recombinant human Hsp90 alpha protein (Carboxyterminal end) (ab196407)
    Western blot - Recombinant human Hsp90 alpha protein (Carboxyterminal end) (ab196407)
    All lanes : Streptavidin-HRP Western blot

    Lane 1 : ab196407
    Lane 2 : Biotinylated control

    Lysates/proteins at 0.25 µg per lane.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab196407? Please let us know so that we can cite the reference in this datasheet.

ab196407 has not yet been referenced specifically in any publications.

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