Recombinant human Hsp90 alpha protein (Carboxyterminal end) (ab196407)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus, proprietary tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human Hsp90 alpha protein (Carboxyterminal end)
See all Hsp90 alpha proteins and peptides -
Purity
> 90 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MHHHHHHGGGLNDIFEAQKIEWHEEFEGKTLVSVTKEGLELPEDEEEKKK QEEKKTKFENLCKIMKDILEKKVEKVVVSNRLVTSPCCIVTSTYGWTANM ERIMKAQALRDNSTMGYMAAKKHLEINPDHSIIETLRQKAEADKNDKSVK DLVILLYETALLSSGFSLEDPQTHANRIYRMIKLGLGIDEDDPTADDTSA AVTEEMPPLEGDDDTSRMEEVD -
Predicted molecular weight
25 kDa including tags -
Amino acids
535 to 732 -
Tags
His tag N-Terminus , proprietary tag N-Terminus -
Additional sequence information
NM_005348. Expressed in an E. coli expression system and enzymatically Biotin-labeled using Avi-tag ™ technology.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab196407 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.64% Tris HCl, 0.64% Sodium chloride, 0.017% Potassium chloride, 0.04% Tween, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- EL52
- epididymis luminal secretory protein 52
- Heat shock 86 kDa
see all -
Function
Molecular chaperone. Has ATPase activity. -
Sequence similarities
Belongs to the heat shock protein 90 family. -
Post-translational
modificationsISGylated. -
Cellular localization
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
Images
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Functional Studies - Recombinant human Hsp90 alpha protein (Carboxyterminal end) (ab196407)
Hsp90 alpha protein (Carboxyterminal end):PPID binding activity measured using Hsp90 alpha protein (Carboxyterminal end) a Inhibitor Screening Assay Kit,
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SDS-PAGE - Recombinant human Hsp90 alpha protein (Carboxyterminal end) (ab196407)4-20% SDS-PAGE analysis of ab196407 at 2 µg stained with Coomassie Blue.
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Western blot - Recombinant human Hsp90 alpha protein (Carboxyterminal end) (ab196407)All lanes : Streptavidin-HRP Western blot
Lane 1 : ab196407
Lane 2 : Biotinylated control
Lysates/proteins at 0.25 µg per lane.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab196407 has not yet been referenced specifically in any publications.
