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  1. Link

    recombinant-human-hsp90-alpha-protein-his-tag-ab48801.pdf

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Signal Transduction Protein Trafficking Chaperones Heat Shock Proteins
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Recombinant Human Hsp90 alpha protein (His tag) (ab48801)

  • Datasheet
Submit a review Q&A (5)References (3)

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SDS-PAGE - Recombinant Human Hsp90 alpha protein (His tag) (ab48801)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 90% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE

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    Description

    • Product name

      Recombinant Human Hsp90 alpha protein (His tag)
      See all Hsp90 alpha proteins and peptides
    • Purity

      > 90 % SDS-PAGE.
      Purified to apparent homogeneity by using conventional column chromatography techniques
    • Expression system

      Escherichia coli
    • Accession

      P07900
    • Protein length

      Full length protein
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Human
      • Sequence

        MGSSHHHHHH SSGLVPRGSH MPEETQTQDQ PMEEEEVETF AFQAEIAQLM SLIINTFYSN KEIFLRELIS NSSDALDKIR YESLTDPSKL DSGKELHINL IPNKQDRTLT IVDTGIGMTK ADLINNLGTI AKSGTKAFME ALQAGADISM IGQFGVGFYS AYLVAEKVTV ITKHNDDEQY AWESSAGGSF TVRTDTGEPM GRGTKVILHL KEDQTEYLEE RRIKEIVKKH SQFIGYPITL FVEKERDKEV SDDEAEEKED KEEEKEKEEK ESEDKPEIED VGSDEEEEKK DGDKKKKKKI KEKYIDQEEL NKTKPIWTRN PDDITNEEYG EFYKSLTNDW EDHLAVKHFS VEGQLEFRAL LFVPRRAPFD LFENRKKKNN IKLYVRRVFI MDNCEELIPE YLNFIRGVVD SEDLPLNISR EMLQQSKILK VIRKNLVKKC LELFTELAED KENYKKFYEQ FSKNIKLGIH EDSQNRKKLS ELLRYYTSAS GDEMVSLKDY CTRMKENQKH IYYITGETKD QVANSAFVER LRKHGLEVIY MIEPIDEYCV QQLKEFEGKT LVSVTKEGLE LPEDEEEKKK QEEKKTKFEN LCKIMKDILE KKVEKVVVSN RLVTSPCCIV TSTYGWTANM ERIMKAQALR DNSTMGYMAA KKHLEINPDH SIIETLRQKA EADKNDKSVK DLVILLYETA LLSSGFSLEDPQTHANRIYR MIKLGLGIDE DDPTADDTSA AVTEEMPPLE GDDDTSRMEE VD
      • Predicted molecular weight

        87 kDa
      • Amino acids

        1 to 732
      • Tags

        His tag N-Terminus

    Associated products

    • Related Products

      • Anti-Hsp90 antibody [AC88] (ab13492)

    Specifications

    Our Abpromise guarantee covers the use of ab48801 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

      pH: 7.40
      Constituents: 0.316% Tris HCl, 0.58% Sodium chloride

    General Info

    • Alternative names

      • EL52
      • epididymis luminal secretory protein 52
      • Heat shock 86 kDa
      • heat shock 90kD protein 1, alpha
      • Heat shock 90kD protein 1, alpha like 4
      • heat shock 90kD protein, alpha-like 4
      • Heat shock 90kDa protein 1 alpha
      • Heat shock protein 90kDa alpha (cytosolic) class A member 1
      • Heat shock protein HSP 90-alpha
      • HS90A_HUMAN
      • HSP 86
      • HSP86
      • Hsp89
      • HSP89A
      • Hsp90
      • HSP90A
      • HSP90AA1
      • HSP90ALPHA
      • HSP90N
      • HSPC1
      • HSPCA
      • HSPCAL1
      • HSPCAL4
      • HSPN
      • LAP 2
      • LAP2
      • lipopolysaccharide-associated protein 2
      • LPS-associated protein 2
      • Renal carcinoma antigen NY-REN-38
      see all
    • Function

      Molecular chaperone. Has ATPase activity.
    • Sequence similarities

      Belongs to the heat shock protein 90 family.
    • Post-translational
      modifications

      ISGylated.
    • Cellular localization

      Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Target information above from: UniProt accession P07900 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human Hsp90 alpha protein (His tag) (ab48801)
      SDS-PAGE - Recombinant Human Hsp90 alpha protein (His tag) (ab48801)
      ab48801 run on a 10% SDS-PAGE gel (Lane 2) with molecular weight markers (Lane 1).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (3)

    Publishing research using ab48801? Please let us know so that we can cite the reference in this datasheet.

    ab48801 has been referenced in 3 publications.

    • Huo Y  et al. Delivering bioactive cyclic peptides that target Hsp90 as prodrugs. J Enzyme Inhib Med Chem 34:728-739 (2019). PubMed: 30822267
    • Moriya C  et al. PRDM14 directly interacts with heat shock proteins HSP90a and glucose-regulated protein 78. Cancer Sci 109:373-383 (2018). PubMed: 29178343
    • Davis AL  et al. The quinone methide aurin is a heat shock response inducer that causes proteotoxic stress and Noxa-dependent apoptosis in malignant melanoma cells. J Biol Chem 290:1623-38 (2015). PubMed: 25477506

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-5 of 5 Abreviews or Q&A

    Question

    Dear xxxx
    Thank you for your kindness!

    The purchase number is xxxxx. Please let me know when I can have the new protein. I will also let you know the result of my assay.

    Have a good weekend

    Read More

    Abcam community

    Verified customer

    Asked on Apr 27 2012

    Answer

    Thank you for confirming that.

    I have arranged for ab80369 to be sent to you. Unfortunately it is currently not in stock and is likely to take until the 10th of May to reach you. I am sorry for any inconvenience this caused you.

    I would be very interested to hear how your assay goes, I will be keeping my fingers crossed. If you have any further questions or any problems in receiving the protein please do let me know.

    Have a nice weekend.

    Read More

    Abcam Scientific Support

    Answered on Apr 27 2012

    Question

    Thank you for your quick reply!

    I am disappointed about what you said. You understood exactly what I need and the purchase was based on the information you provided. I don’t know why you decided not to refund this?

    Regards

    Read More

    Abcam community

    Verified customer

    Asked on Apr 27 2012

    Answer

    I am sorry that you are disappointed. I understood from our initial phone call that you had already purchased ab48801 and I therefore did not make the recommendation of ab80369 as an alternative immediately. As I said in my initial email to you, we have not tested ab48801 ourselves for its biological activity and cannot therefore say whether it would work or not. It is regrettable that you found it not to work in your assay.

    As a gesture of goodwill, I am willing to give you a vial of ab80369 to try. I am sorry but even though this has been found to be active in binding geldanamycin I cannot guarantee that is will work in your assay. If you would like me to do this please could you supply me with your order number and I will have this arranged.

    I look forward to hearing how you would like to proceed.

    Read More

    Abcam Scientific Support

    Answered on Apr 27 2012

    Question

    Dear xxxx,

    I bought the batch you below. However, I could not see any activity. Please see the file attached.

    Is there any chance I can get the refund or you can give me some active Hsp90 instead?

    Best wishes

    Read More

    Abcam community

    Verified customer

    Asked on Apr 27 2012

    Answer

    I am sorry to hear that you found the protein Hsp90 alpha(ab48801) not to be active in your assay. As I mentioned in my previous email, as we have not performed any activity assays ourselves, we could not therefore be sure that the protein would be active and arenot able to guarantee this.

    We have an alternative protein Hsp90 alpha protein (Active), ab80369 which may be of interest to you. This has been shown to be able to bind geldanamycin with a Kd of 8 nM, howeverI could not be surethat it would work in yourspecificassay.

    I hope this information has been of some help. If you require any further information please do not hesitate to ask.

    Read More

    Abcam Scientific Support

    Answered on Apr 27 2012

    Question

    Phone call requesting information on if the protein Hsp90 alpha protein (His tag) is active?

    Read More

    Abcam community

    Verified customer

    Asked on Apr 17 2012

    Answer

    Thank you for contacting us in regards to Hsp90 alpha protein (His tag) (ab48801).

    I have contacted the source of this protein and unfortunately they ahve informed me thattheprotein’s biological activity has not beenassessed asyet, and we can not provide aspecific date when this will be perfomred. However, I would say that the protein has not beenexposed to any denaturing agents, and thereforeit is entirely possible that it is bioactive.

    I hope this information has been of some help. If you require any further information please do not hestiate to contact us again.

    Read More

    Abcam Scientific Support

    Answered on Apr 17 2012

    Question

    Hi, Before ordering ab48801 I would like to know if you have verified either ATPase or functional activity of your Hsp90 protein stock? Regards    

    Read More

    Abcam community

    Verified customer

    Asked on Dec 08 2011

    Answer

    Thank you for contacting us. The source of the protein informed me that, regrettably, they haven’t tested the activity of the Hsp 90 (ab48801). There are no test scheduled for this particular protein in the near future because it requires radioactive labeling. I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Dec 08 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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