Recombinant Human Hsp90 alpha protein (His tag) (ab48801)

Submit a review Q&A (5)References (2)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
      P07900
    • Species
      Human
    • Sequence
      MGSSHHHHHH SSGLVPRGSH MPEETQTQDQ PMEEEEVETF AFQAEIAQLM SLIINTFYSN KEIFLRELIS NSSDALDKIR YESLTDPSKL DSGKELHINL IPNKQDRTLT IVDTGIGMTK ADLINNLGTI AKSGTKAFME ALQAGADISM IGQFGVGFYS AYLVAEKVTV ITKHNDDEQY AWESSAGGSF TVRTDTGEPM GRGTKVILHL KEDQTEYLEE RRIKEIVKKH SQFIGYPITL FVEKERDKEV SDDEAEEKED KEEEKEKEEK ESEDKPEIED VGSDEEEEKK DGDKKKKKKI KEKYIDQEEL NKTKPIWTRN PDDITNEEYG EFYKSLTNDW EDHLAVKHFS VEGQLEFRAL LFVPRRAPFD LFENRKKKNN IKLYVRRVFI MDNCEELIPE YLNFIRGVVD SEDLPLNISR EMLQQSKILK VIRKNLVKKC LELFTELAED KENYKKFYEQ FSKNIKLGIH EDSQNRKKLS ELLRYYTSAS GDEMVSLKDY CTRMKENQKH IYYITGETKD QVANSAFVER LRKHGLEVIY MIEPIDEYCV QQLKEFEGKT LVSVTKEGLE LPEDEEEKKK QEEKKTKFEN LCKIMKDILE KKVEKVVVSN RLVTSPCCIV TSTYGWTANM ERIMKAQALR DNSTMGYMAA KKHLEINPDH SIIETLRQKA EADKNDKSVK DLVILLYETA LLSSGFSLEDPQTHANRIYR MIKLGLGIDE DDPTADDTSA AVTEEMPPLE GDDDTSRMEE VD
    • Amino acids
      1 to 732
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab48801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity
    > 90 % SDS-PAGE.
    Purified to apparent homogeneity by using conventional column chromatography techniques
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    pH: 7.40
    Constituents: 0.316% Tris HCl, 0.58% Sodium chloride

General Info

  • Alternative names
    • EL52
    • epididymis luminal secretory protein 52
    • Heat shock 86 kDa
    • heat shock 90kD protein 1, alpha
    • Heat shock 90kD protein 1, alpha like 4
    • heat shock 90kD protein, alpha-like 4
    • Heat shock 90kDa protein 1 alpha
    • Heat shock protein 90kDa alpha (cytosolic) class A member 1
    • Heat shock protein HSP 90-alpha
    • HS90A_HUMAN
    • HSP 86
    • HSP86
    • Hsp89
    • HSP89A
    • Hsp90
    • HSP90A
    • HSP90AA1
    • HSP90ALPHA
    • HSP90N
    • HSPC1
    • HSPCA
    • HSPCAL1
    • HSPCAL4
    • HSPN
    • LAP 2
    • LAP2
    • lipopolysaccharide-associated protein 2
    • LPS-associated protein 2
    • Renal carcinoma antigen NY-REN-38
    see all
  • Function
    Molecular chaperone. Has ATPase activity.
  • Sequence similarities
    Belongs to the heat shock protein 90 family.
  • Post-translational
    modifications
    ISGylated.
  • Cellular localization
    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human Hsp90 alpha protein (His tag) (ab48801)
    SDS-PAGE - Recombinant Human Hsp90 alpha protein (His tag) (ab48801)
    ab48801 run on a 10% SDS-PAGE gel (Lane 2) with molecular weight markers (Lane 1).

References

This product has been referenced in:
  • Moriya C  et al. PRDM14 directly interacts with heat shock proteins HSP90a and glucose-regulated protein 78. Cancer Sci 109:373-383 (2018). Read more (PubMed: 29178343) »
  • Davis AL  et al. The quinone methide aurin is a heat shock response inducer that causes proteotoxic stress and Noxa-dependent apoptosis in malignant melanoma cells. J Biol Chem 290:1623-38 (2015). Read more (PubMed: 25477506) »
See all 2 Publications for this product

Publishing research using ab48801? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-5 of 5 Abreviews or Q&A

Question

Dear xxxx
Thank you for your kindness!

The purchase number is xxxxx. Please let me know when I can have the new protein. I will also let you know the result of my assay.

Have a good weekend

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Answer

Thank you for confirming that.

I have arranged for ab80369 to be sent to you. Unfortunately it is currently not in stock and is likely to take until the 10th of May to reach you. I am sorry for any inconvenience this caused you.

I would be very interested to hear how your assay goes, I will be keeping my fingers crossed. If you have any further questions or any problems in receiving the protein please do let me know.

Have a nice weekend.

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Question

Thank you for your quick reply!

I am disappointed about what you said. You understood exactly what I need and the purchase was based on the information you provided. I don’t know why you decided not to refund this?

Regards

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Answer

I am sorry that you are disappointed. I understood from our initial phone call that you had already purchased ab48801 and I therefore did not make the recommendation of ab80369 as an alternative immediately. As I said in my initial email to you, we have not tested ab48801 ourselves for its biological activity and cannot therefore say whether it would work or not. It is regrettable that you found it not to work in your assay.

As a gesture of goodwill, I am willing to give you a vial of ab80369 to try. I am sorry but even though this has been found to be active in binding geldanamycin I cannot guarantee that is will work in your assay. If you would like me to do this please could you supply me with your order number and I will have this arranged.

I look forward to hearing how you would like to proceed.

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Question

Dear xxxx,

I bought the batch you below. However, I could not see any activity. Please see the file attached.

Is there any chance I can get the refund or you can give me some active Hsp90 instead?

Best wishes

Read More
Answer

I am sorry to hear that you found the protein Hsp90 alpha(ab48801) not to be active in your assay. As I mentioned in my previous email, as we have not performed any activity assays ourselves, we could not therefore be sure that the protein would be active and arenot able to guarantee this.

We have an alternative protein Hsp90 alpha protein (Active), ab80369 which may be of interest to you. This has been shown to be able to bind geldanamycin with a Kd of 8 nM, howeverI could not be surethat it would work in yourspecificassay.

I hope this information has been of some help. If you require any further information please do not hesitate to ask.

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Question

Phone call requesting information on if the protein Hsp90 alpha protein (His tag) is active?

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Answer

Thank you for contacting us in regards to Hsp90 alpha protein (His tag) (ab48801).

I have contacted the source of this protein and unfortunately they ahve informed me thattheprotein’s biological activity has not beenassessed asyet, and we can not provide aspecific date when this will be perfomred. However, I would say that the protein has not beenexposed to any denaturing agents, and thereforeit is entirely possible that it is bioactive.

I hope this information has been of some help. If you require any further information please do not hestiate to contact us again.

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Question

Hi, Before ordering ab48801 I would like to know if you have verified either ATPase or functional activity of your Hsp90 protein stock? Regards    

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Answer

Thank you for contacting us. The source of the protein informed me that, regrettably, they haven’t tested the activity of the Hsp 90 (ab48801). There are no test scheduled for this particular protein in the near future because it requires radioactive labeling. I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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