Key features and details
- Expression system: Insect cells
- Purity: > 70% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Product nameRecombinant human htrA1 protein
See all htrA1 proteins and peptides
Biological activityProteolytic activity of ab134441 was documented by digestion of ß- casein. 0.5 mg ß-casein/ml are completely digested by 5 µg/ml ab134441 within 3 hours at 37°C.
Purity> 70 % SDS-PAGE.
ab134441 was purified from insect cell culture supernatants.
Expression systemInsect cells
Protein lengthFull length protein
Predicted molecular weight50 kDa including tags
Amino acids1 to 480
TagsHis tag C-Terminus
Our Abpromise guarantee covers the use of ab134441 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Preservative: 0.34% Imidazole
Constituents: 0.05% Brij, 0.05% Calcium chloride, 0.79% Tris HCl, 0.88% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- High-temperature requirement A serine peptidase 1
FunctionProtease that regulate the availability of nsulin-like growth factors (IGFs) by cleaving IGF-binding proteins. Represses signaling by TGF-beta family members.
Tissue specificityExpressed in a variety of tissues, with strongest expression in placenta.
Involvement in diseaseVariations in the promoter region of HTRA1 are the cause of susceptibility to age-related macular degeneration type 7 (ARMD7) [MIM:610149]. ARMD is the leading cause of vision loss and blindness among older individuals in the developed word. It is classified as either dry (nonneovascular) or wet (neovascular). ARMD7 is a wet form, in which new blood vessels form and break beneath the retina. This leakage causes permanent damage to surrounding retinal tissue, distorting and destroying central vision. Wet ARMD is more prevalent among Asians than Caucasians.
Defects in HTRA1 are the cause of cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy (CARASIL) [MIM:600142]. CARASIL is characterized by nonhypertensive cerebral small-vessel arteriopathy with subcortical infarcts, alopecia, and spondylosis, with an onset in early adulthood. On neuropathological examination, arteriosclerosis associated with intimal thickening and dense collagen fibers, loss of vascular smooth-muscle cells, and hyaline degeneration of the tunica media has been observed in cerebral small arteries.
Sequence similaritiesBelongs to the peptidase S1B family.
Contains 1 IGFBP N-terminal domain.
Contains 1 Kazal-like domain.
Contains 1 PDZ (DHR) domain.
- Information by UniProt
SDS-PAGE analysis of ab134441 (2µg).
Hydrolysis of ß-Casein by ab134441. ß-Casein (0.5 mg/ml) was incubated in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5 mM CaCl2 without or with ab134441 (5 µg/ml). After various time intervals aliquots of the reaction mixtures were withdrawn and analyzed by SDS-PAGE.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab134441 has been referenced in 1 publication.
- Lin MK et al. HTRA1, an age-related macular degeneration protease, processes extracellular matrix proteins EFEMP1 and TSP1. Aging Cell N/A:e12710 (2018). PubMed: 29730901