Overview

Description

  • Nature

    Recombinant
  • Source

    Insect cells
  • Amino Acid Sequence
    • Accession
    • Species

      Human
    • Molecular weight

      50 kDa including tags
    • Amino acids

      1 to 480
    • Tags

      His tag C-Terminus

Specifications

Our Abpromise guarantee covers the use of ab134441 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    Proteolytic activity of ab134441 was documented by digestion of ß- casein. 0.5 mg ß-casein/ml are completely digested by 5 µg/ml ab134441 within 3 hours at 37°C.
  • Applications

    SDS-PAGE

    Functional Studies

  • Purity

    > 70 % SDS-PAGE.
    ab134441 was purified from insect cell culture supernatants.
  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Preservative: 0.34% Imidazole
    Constituents: 0.05% Brij, 0.06% Calcium chloride, 0.79% Tris HCl, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • ARMD7
    • CARASIL
    • High-temperature requirement A serine peptidase 1
    • HtrA
    • HtrA serine peptidase 1
    • HTRA1
    • HTRA1_HUMAN
    • IGFBP5 protease
    • L56
    • ORF480
    • protease serine 11
    • Protease serine 11 (IGF binding)
    • PRSS11
    • Serine protease 11
    • Serine protease HTRA1
    • Serine protease HTRA1 precursor
    see all
  • Function

    Protease that regulate the availability of nsulin-like growth factors (IGFs) by cleaving IGF-binding proteins. Represses signaling by TGF-beta family members.
  • Tissue specificity

    Expressed in a variety of tissues, with strongest expression in placenta.
  • Involvement in disease

    Variations in the promoter region of HTRA1 are the cause of susceptibility to age-related macular degeneration type 7 (ARMD7) [MIM:610149]. ARMD is the leading cause of vision loss and blindness among older individuals in the developed word. It is classified as either dry (nonneovascular) or wet (neovascular). ARMD7 is a wet form, in which new blood vessels form and break beneath the retina. This leakage causes permanent damage to surrounding retinal tissue, distorting and destroying central vision. Wet ARMD is more prevalent among Asians than Caucasians.
    Defects in HTRA1 are the cause of cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy (CARASIL) [MIM:600142]. CARASIL is characterized by nonhypertensive cerebral small-vessel arteriopathy with subcortical infarcts, alopecia, and spondylosis, with an onset in early adulthood. On neuropathological examination, arteriosclerosis associated with intimal thickening and dense collagen fibers, loss of vascular smooth-muscle cells, and hyaline degeneration of the tunica media has been observed in cerebral small arteries.
  • Sequence similarities

    Belongs to the peptidase S1B family.
    Contains 1 IGFBP N-terminal domain.
    Contains 1 Kazal-like domain.
    Contains 1 PDZ (DHR) domain.
  • Cellular localization

    Secreted.
  • Information by UniProt

Images

  • SDS-PAGE analysis of ab134441 (2µg).
  • Hydrolysis of ß-Casein by ab134441. ß-Casein (0.5 mg/ml) was incubated in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5 mM CaCl2 without or with ab134441 (5 µg/ml). After various time intervals aliquots of the reaction mixtures were withdrawn and analyzed by SDS-PAGE.

References

This product has been referenced in:

  • Lin MK  et al. HTRA1, an age-related macular degeneration protease, processes extracellular matrix proteins EFEMP1 and TSP1. Aging Cell N/A:e12710 (2018). Read more (PubMed: 29730901) »
See 1 Publication for this product

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