Recombinant human HtrA3 protein (ab134450)
- Datasheet
- References
- Protocols
Description
-
Product name
Recombinant human HtrA3 protein
See all HtrA3 proteins and peptides -
Biological activity
Proteolytic activity of ab134450 was documented by digestion of ß- casein. 0.5 mg ß-casein/ml are completely digested by 50 µg/ml HtrA3 within 2 hours at 37°C. -
Purity
> 70 % SDS-PAGE. -
Expression system
Insect cells -
Accession
-
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Predicted molecular weight
48 kDa including tags -
Amino acids
1 to 453 -
Tags
His tag C-Terminus
-
Associated products
-
Related Products
Specifications
Our Abpromise guarantee covers the use of ab134450 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
Applications
SDS-PAGE
Inhibition Assay
Functional Studies
-
Form
Liquid -
Concentration information loading...
Preparation and Storage
-
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Preservative: 0.34% Imidazole
Constituents: 0.05% Brij, 0.05% Calcium chloride, 0.79% Tris HCl, 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
-
Alternative names
- High temperature requirement factor A3
- High-temperature requirement factor A3
- HtrA serine peptidase 3
see all -
Function
Probable serine protease. -
Tissue specificity
Widely expressed, with highest levels in both adult and fetal heart, ovary, uterus placenta, and bladder. Isoform 1 is predominant in heart and skeletal muscle, whereas isoform 2 is predominant in placenta and kidney. -
Sequence similarities
Belongs to the peptidase S1B family.
Contains 1 IGFBP N-terminal domain.
Contains 1 Kazal-like domain.
Contains 1 PDZ (DHR) domain. -
Cellular localization
Secreted. - Information by UniProt
Images
-
SDS-PAGE - Recombinant human HtrA3 protein (ab134450)SDS-PAGE analysis of ab134450 (1µg). Minor bands of HtrA3 fragments may be visible in the enzyme preparation. -
SDS-PAGE - Recombinant human HtrA3 protein (ab134450)Hydrolysis of beta-Casein by ab134450. ß-Casein (0.5 mg/ml) was incubated in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.5 mM CaCl2 with or without HtrA3 (50 µg/ml). After indicated time intervals 10 µl aliquots of the reaction mixtures were withdrawn and analyzed by SDS-PAGE.
References
ab134450 has not yet been referenced specifically in any publications.
