Recombinant human HtrA3 protein (ab134450)
Key features and details
- Expression system: Insect cells
- Purity: > 70% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Inhibition Assay, Functional Studies
Description
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Product name
Recombinant human HtrA3 protein
See all HtrA3 proteins and peptides -
Biological activity
Proteolytic activity of ab134450 was documented by digestion of ß- casein. 0.5 mg ß-casein/ml are completely digested by 50 µg/ml HtrA3 within 2 hours at 37°C. -
Purity
> 70 % SDS-PAGE. -
Expression system
Insect cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
48 kDa including tags -
Amino acids
1 to 453 -
Tags
His tag C-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab134450 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Inhibition Assay
Functional Studies
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.79% Tris HCl, 0.88% Sodium chloride, 50% GlycerolThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- High temperature requirement factor A3
- High-temperature requirement factor A3
- HtrA serine peptidase 3
see all -
Function
Probable serine protease. -
Tissue specificity
Widely expressed, with highest levels in both adult and fetal heart, ovary, uterus placenta, and bladder. Isoform 1 is predominant in heart and skeletal muscle, whereas isoform 2 is predominant in placenta and kidney. -
Sequence similarities
Belongs to the peptidase S1B family.
Contains 1 IGFBP N-terminal domain.
Contains 1 Kazal-like domain.
Contains 1 PDZ (DHR) domain. -
Cellular localization
Secreted. - Information by UniProt
Images
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SDS-PAGE - Recombinant human HtrA3 protein (ab134450)SDS-PAGE analysis of ab134450 (1µg). Minor bands of HtrA3 fragments may be visible in the enzyme preparation.
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SDS-PAGE - Recombinant human HtrA3 protein (ab134450)Hydrolysis of beta-Casein by ab134450. ß-Casein (0.5 mg/ml) was incubated in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.5 mM CaCl2 with or without HtrA3 (50 µg/ml). After indicated time intervals 10 µl aliquots of the reaction mixtures were withdrawn and analyzed by SDS-PAGE.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab134450 has not yet been referenced specifically in any publications.
