Key features and details
- Expression system: Insect cells
- Purity: > 70% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Inhibition Assay, Functional Studies
Product nameRecombinant human HtrA3 protein
See all HtrA3 proteins and peptides
Biological activityProteolytic activity of ab134450 was documented by digestion of ß- casein. 0.5 mg ß-casein/ml are completely digested by 50 µg/ml HtrA3 within 2 hours at 37°C.
Purity> 70 % SDS-PAGE.
Expression systemInsect cells
Protein lengthFull length protein
Predicted molecular weight48 kDa including tags
Amino acids1 to 453
TagsHis tag C-Terminus
Our Abpromise guarantee covers the use of ab134450 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.79% Tris HCl, 0.88% Sodium chloride, 50% Glycerol
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- High temperature requirement factor A3
- High-temperature requirement factor A3
- HtrA serine peptidase 3
FunctionProbable serine protease.
Tissue specificityWidely expressed, with highest levels in both adult and fetal heart, ovary, uterus placenta, and bladder. Isoform 1 is predominant in heart and skeletal muscle, whereas isoform 2 is predominant in placenta and kidney.
Sequence similaritiesBelongs to the peptidase S1B family.
Contains 1 IGFBP N-terminal domain.
Contains 1 Kazal-like domain.
Contains 1 PDZ (DHR) domain.
- Information by UniProt
SDS-PAGE analysis of ab134450 (1µg). Minor bands of HtrA3 fragments may be visible in the enzyme preparation.
Hydrolysis of beta-Casein by ab134450. ß-Casein (0.5 mg/ml) was incubated in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.5 mM CaCl2 with or without HtrA3 (50 µg/ml). After indicated time intervals 10 µl aliquots of the reaction mixtures were withdrawn and analyzed by SDS-PAGE.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab134450 has not yet been referenced specifically in any publications.