Recombinant human htrA4 protein (ab134446)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies, Inhibition Assay
Description
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Product name
Recombinant human htrA4 protein
See all htrA4 proteins and peptides -
Biological activity
Proteolytic activity of ab134446 was documented by digestion of ß- casein. 0.5 mg ß-casein/ml are completely digested by 10 µg/ml HtrA4 within 24 hours at 37°C. -
Purity
> 90 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MRLGKVPAVPVQWGNCGDTGTRSAGPLRRNYNFIAAVVEKVAPSVVHVQL WGRLLHGSRLVPVYSGSGFIVSEDGLIITNAHVVRNQQWIEVVLQNGARY EAVVKDIDLKLDLAVIKIESNAELPVLMLGRSSDLRAGEFVVALGSPFSL QNTATAGIVSTKQRGGKELGMKDSDMDYVQIDATINYGNSGGPLVNLDGD VIGVNSLRVTDGISFAIPSDRVRQFLAEYHEHQMKGKAFSNKKYLGLQML SLTVPLSEELKMHYPDFPDVSSGVYVCKVVEGTAAQSSGLRDHDVIVNIN GKPITTTTDVVKALDSDSLSMAVLRGKDNLLLTVIPETINHHHHHH -
Predicted molecular weight
38 kDa including tags -
Amino acids
138 to 476 -
Tags
His tag C-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab134446 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
Inhibition Assay
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Form
Liquid -
Additional notes
Upon freezing, a faint precipitate may form which contains htrA4. This precipitate was shown to contain catalytically active htrA4 in a casein digest. Therefore, we recommend vortexing ab134446 before use. -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Preservative: 0.816% Imidazole
Constituents: 0.64% Tris HCl, 15% Glycerol, 0.704% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- FLJ90724
- High temperature requirement protean A4
- High-temperature requirement factor A4
see all -
Function
Serine protease. -
Sequence similarities
Belongs to the peptidase S1B family.
Contains 1 IGFBP N-terminal domain.
Contains 1 Kazal-like domain.
Contains 1 PDZ (DHR) domain. -
Cellular localization
Secreted. - Information by UniProt
Images
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SDS-PAGE analysis of ab134446 (1µg). Due to minor N-terminal truncation of the protein it may appear as a double band.
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Hydrolysis of ß-Casein by ab134446. ß-Casein (0.5 mg/ml) was incubated in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.5 mM CaCl2 with or without htrA4 (10 µg/ml). After indicated time intervals 15 µl aliquots of the reaction mixtures were withdrawn and analyzed by SDS-PAGE.
Lane 1: BSA-only control, 0 hours
Lane 2: BSA-only control, 24 hours
Lane 3: 1µg ab134446, 0 hours
Lane 4: 1µg ab134446, 6 hours
Lane 5: 1µg ab134446, 24 hours
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab134446 has not yet been referenced specifically in any publications.