Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies, Inhibition Assay
Product nameRecombinant human htrA4 protein
See all htrA4 proteins and peptides
Biological activityProteolytic activity of ab134446 was documented by digestion of ß- casein. 0.5 mg ß-casein/ml are completely digested by 10 µg/ml HtrA4 within 24 hours at 37°C.
Purity> 90 % SDS-PAGE.
Expression systemEscherichia coli
Protein lengthProtein fragment
SequenceMRLGKVPAVPVQWGNCGDTGTRSAGPLRRNYNFIAAVVEKVAPSVVHVQL WGRLLHGSRLVPVYSGSGFIVSEDGLIITNAHVVRNQQWIEVVLQNGARY EAVVKDIDLKLDLAVIKIESNAELPVLMLGRSSDLRAGEFVVALGSPFSL QNTATAGIVSTKQRGGKELGMKDSDMDYVQIDATINYGNSGGPLVNLDGD VIGVNSLRVTDGISFAIPSDRVRQFLAEYHEHQMKGKAFSNKKYLGLQML SLTVPLSEELKMHYPDFPDVSSGVYVCKVVEGTAAQSSGLRDHDVIVNIN GKPITTTTDVVKALDSDSLSMAVLRGKDNLLLTVIPETINHHHHHH
Predicted molecular weight38 kDa including tags
Amino acids138 to 476
TagsHis tag C-Terminus
Our Abpromise guarantee covers the use of ab134446 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Additional notesUpon freezing, a faint precipitate may form which contains htrA4. This precipitate was shown to contain catalytically active htrA4 in a casein digest. Therefore, we recommend vortexing ab134446 before use.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Preservative: 0.816% Imidazole
Constituents: 0.64% Tris HCl, 15% Glycerol, 0.704% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- High temperature requirement protean A4
- High-temperature requirement factor A4
Sequence similaritiesBelongs to the peptidase S1B family.
Contains 1 IGFBP N-terminal domain.
Contains 1 Kazal-like domain.
Contains 1 PDZ (DHR) domain.
- Information by UniProt
SDS-PAGE analysis of ab134446 (1µg). Due to minor N-terminal truncation of the protein it may appear as a double band.
Hydrolysis of ß-Casein by ab134446. ß-Casein (0.5 mg/ml) was incubated in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.5 mM CaCl2 with or without htrA4 (10 µg/ml). After indicated time intervals 15 µl aliquots of the reaction mixtures were withdrawn and analyzed by SDS-PAGE.
Lane 1: BSA-only control, 0 hours
Lane 2: BSA-only control, 24 hours
Lane 3: 1µg ab134446, 0 hours
Lane 4: 1µg ab134446, 6 hours
Lane 5: 1µg ab134446, 24 hours
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab134446 has not yet been referenced specifically in any publications.