Recombinant human htrA4 protein (ab134446)

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SDS-PAGE - Recombinant human htrA4 protein (ab134446)
  • SDS-PAGE - Recombinant human htrA4 protein (ab134446)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 90% SDS-PAGE
  • Active: Yes
  • Tags: His tag C-Terminus
  • Suitable for: SDS-PAGE, Functional Studies, Inhibition Assay

Description

  • Product name

    Recombinant human htrA4 protein
    See all htrA4 proteins and peptides

  • Biological activity

    Proteolytic activity of ab134446 was documented by digestion of ß- casein. 0.5 mg ß-casein/ml are completely digested by 10 µg/ml HtrA4 within 24 hours at 37°C.

  • Purity

    > 90 % SDS-PAGE.

  • Expression system

    Escherichia coli

  • Accession

    P83105

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MRLGKVPAVPVQWGNCGDTGTRSAGPLRRNYNFIAAVVEKVAPSVVHVQL WGRLLHGSRLVPVYSGSGFIVSEDGLIITNAHVVRNQQWIEVVLQNGARY EAVVKDIDLKLDLAVIKIESNAELPVLMLGRSSDLRAGEFVVALGSPFSL QNTATAGIVSTKQRGGKELGMKDSDMDYVQIDATINYGNSGGPLVNLDGD VIGVNSLRVTDGISFAIPSDRVRQFLAEYHEHQMKGKAFSNKKYLGLQML SLTVPLSEELKMHYPDFPDVSSGVYVCKVVEGTAAQSSGLRDHDVIVNIN GKPITTTTDVVKALDSDSLSMAVLRGKDNLLLTVIPETINHHHHHH

    • Predicted molecular weight

      38 kDa including tags

    • Amino acids

      138 to 476

    • Tags

      His tag C-Terminus

Specifications

Our Abpromise guarantee covers the use of ab134446 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

    Inhibition Assay

  • Form

    Liquid

  • Additional notes

    Upon freezing, a faint precipitate may form which contains htrA4. This precipitate was shown to contain catalytically active htrA4 in a casein digest. Therefore, we recommend vortexing ab134446 before use.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Preservative: 0.816% Imidazole
    Constituents: 0.64% Tris HCl, 15% Glycerol, 0.704% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • FLJ90724
    • High temperature requirement protean A4
    • High-temperature requirement factor A4
    • HTRA 4
    • HtrA serine peptidase 4
    • HTRA4
    • HTRA4_HUMAN
    • Probable serine protease HTRA4
    • Serine protease HTRA4
    see all

  • Function

    Serine protease.

  • Sequence similarities

    Belongs to the peptidase S1B family.
    Contains 1 IGFBP N-terminal domain.
    Contains 1 Kazal-like domain.
    Contains 1 PDZ (DHR) domain.

  • Cellular localization

    Secreted.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant human htrA4 protein (ab134446)
    SDS-PAGE - Recombinant human htrA4 protein (ab134446)
    SDS-PAGE analysis of ab134446 (1µg). Due to minor N-terminal truncation of the protein it may appear as a double band.
  • SDS-PAGE - Recombinant human htrA4 protein (ab134446)
    SDS-PAGE - Recombinant human htrA4 protein (ab134446)
    Hydrolysis of ß-Casein by ab134446. ß-Casein (0.5 mg/ml) was incubated in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.5 mM CaCl2 with or without htrA4 (10 µg/ml). After indicated time intervals 15 µl aliquots of the reaction mixtures were withdrawn and analyzed by SDS-PAGE.
    Lane 1: BSA-only control, 0 hours
    Lane 2: BSA-only control, 24 hours
    Lane 3: 1µg ab134446, 0 hours
    Lane 4: 1µg ab134446, 6 hours
    Lane 5: 1µg ab134446, 24 hours

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab134446? Please let us know so that we can cite the reference in this datasheet.

ab134446 has not yet been referenced specifically in any publications.

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