Description

  • Product name

    Recombinant Human HuR / ELAVL1 protein
    See all HuR / ELAVL1 proteins and peptides
  • Purity

    > 80 % SDS-PAGE.

  • Expression system

    Wheat germ
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MSNGYEDHMAEDCRGDIGRTNLIVNYLPQNMTQDELRSLFSSIGEVESAK LIRDKVAGHSLGYGFVNYVTAKDAERAINTLNGLRLQSKTIKVSYARPSS EVIKDANLYISGLPRTMTQKDVEDMFSRFGRIINSRVLVDQTTGLSRGVA FIRFDKRSEAEEAITSFNGHKPPGSSEPITVKFAANPNQNKNVALLSQLY HSPARRFGGPVHHQAQRFRFSPMGVDHMSGLSGVNVPGNASSGWCIFIYN LGQDADEGILWQMFGPFGAVTNVKVIRDFNTNKCKGFGFVTMTNYEEAAM AIASLNGYRLGDKILQVSFKTNKSHK
    • Predicted molecular weight

      63 kDa including tags
    • Amino acids

      1 to 362
    • Tags

      GST tag N-Terminus

Associated products

Specifications

Our Abpromise guarantee covers the use of ab152360 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    ELISA

    Western blot

    SDS-PAGE

  • Form

    Liquid
  • Additional notes

    Protein concentration is above or equal to 0.05 µg/µl.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.31% Glutathione, 0.79% Tris HCl

General Info

  • Alternative names

    • ELAV (embryonic lethal abnormal vision Drosophila) like 1
    • ELAV (embryonic lethal, abnormal vision, Drosophila) like 1 (Hu antigen R)
    • ELAV like 1
    • ELAV like RNA binding protein 1
    • ELAV-like protein 1
    • ELAV1
    • ELAV1_HUMAN
    • Elavl1
    • Embryonic lethal abnormal vision drosophila homolog like 1
    • Hu Antigen R
    • Hu-antigen R
    • Hua
    • HuR
    • MelG
    see all
  • Function

    Involved in 3'-UTR ARE-mediated MYC stabilization. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, HUR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the RRM elav family.
    Contains 3 RRM (RNA recognition motif) domains.
  • Post-translational
    modifications

    Methylated at Arg-217 by CARM1 in macrophages in response to LPS challenge.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt

Images

  • 12.5% SDS-PAGE analysis of ab152360 stained with Coomassie Blue.

References

ab152360 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

The image on the datasheet is a SDS PAGE image rather than a western blot, so the amount of protein loaded for that particular gel is not necessarily applicable to your western blot. For purified protein such as ab152360 we recommend loading 10 - 100 ng per lane. For lysates we recommend loading 20-30 ug of total protein.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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