Recombinant Human Hyaluronidase PH20 protein (ab174000)

Overview

Description

  • Nature
    Recombinant
  • Source
    HEK 293 cells
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      LNFRAPPVIPNVPFLWAWNAPSEFCLGKFDEPLDMSLFSFIGSPRINATG QGVTIFYVDRLGYYPYIDSITGVTVNGGIPQKISLQDHLDKAKKDITFYM PVDNLGMAVIDWEEWRPTWARNWKPKDVYKNRSIELVQQQNVQLSLTEAT EKAKQEFEKAGKDFLVETIKLGKLLRPNHLWGYYLFPDCYNHHYKKPGYN GSCFNVEIKRNDDLSWLWNESTALYPSIYLNTQQSPVAATLYVRNRVREA IRVSKIPDAKSPLPVFAYTRIVFTDQVLKFLSQDELVYTFGETVALGASG IVIWGTLSIMRSMKSCLLLDNYMETILNPYIINVTLAAKMCSQVLCQE QGVCIRKNWNSSDYLHLNPDNFAIQLEKGGKFTVRGKPTLEDLEQFSEKF YCSCYSTLSCKEKADVKDTDAVDVCIADGVCIDAFLKPPMETEEPQIFY
    • Molecular weight
      52 kDa including tags
    • Amino acids
      36 to 482
    • Tags
      His tag C-Terminus
    • Additional sequence information
      GenBank: AAH26163.1

Specifications

Our Abpromise guarantee covers the use of ab174000 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Endotoxin level
    < 1.000 Eu/µg
  • Purity
    > 92 % SDS-PAGE.

  • Form
    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -20°C or -80°C. Reconstitute for long term storage.

    Information available upon request.

  • Reconstitution
    It is recommended to reconstitute the lyophilized protein in sterile deionized water to a final concentration of 1mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.

General Info

  • Alternative names
    • epididymis secretory sperm binding protein Li 96n
    • HEL-S-96n
    • HYA1
    • HYAL PH-20
    • HYAL PH20
    • Hyal-PH20
    • HYAL1
    • HYAL3
    • HYAL5
    • HYALP_HUMAN
    • Hyaluronidase PH-20
    • Hyaluronoglucosaminidase PH-20
    • MGC26532
    • PH-20 Hyaluronidase
    • PH20
    • PH20 Hyaluronidase
    • SPAG15
    • SPAM-1
    • Spam1
    • Sperm adhesion molecule 1
    • sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding)
    • Sperm surface protein PH-20
    • Sperm surface protein PH20
    • zona pellucida binding
    see all
  • Function
    Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.
  • Tissue specificity
    Testis.
  • Sequence similarities
    Belongs to the glycosyl hydrolase 56 family.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localization
    Cell membrane.
  • Information by UniProt

Images

  • SDS-PAGE analysis of reduced ab174000 stained overnight with Coomassie Blue.

    DTT-reduced Protein migrates as 64-66 kDa due to glycosylation.

References

ab174000 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab174000.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up