Recombinant Human ICAM4 protein (ab127252)

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Key features and details

  • Expression system: Escherichia coli
  • Tags: His-DHFR tag N-Terminus
  • Suitable for: SDS-PAGE

Description

  • Product name

    Recombinant Human ICAM4 protein

  • Expression system

    Escherichia coli

  • Accession

    Q9BWR0

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Predicted molecular weight

      18 kDa

    • Amino acids

      43 to 210

    • Tags

      His-DHFR tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab127252 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Lyophilized
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20ºC.

    Constituents: 0.32% Tris HCl, 0.58% Sodium chloride

  • Reconstitution
    Reconstitute with water to desired concentration.

General Info

  • Alternative names

    • CD242
    • CD242 antigen
    • ICAM-4
    • Icam4
    • ICAM4_HUMAN
    • Intercellular adhesion molecule 4
    • Landsteiner Wiener blood group glycoprotein
    • Landsteiner-Wiener blood group glycoprotein
    • LW
    • LW blood group protein
    see all

  • Function

    ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM4 is also a ligand for alpha-4/beta-1 and alpha-V integrins.

  • Tissue specificity

    Erythrocytes.

  • Sequence similarities

    Belongs to the immunoglobulin superfamily. ICAM family.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

  • Post-translational
    modifications

    N- and O-glycosylated.

  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab127252? Please let us know so that we can cite the reference in this datasheet.

ab127252 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Question

Hello!
I have a question about your recombinant proteins that are tagged
with His-DHFR tag (for example product code- ab127252). In the datasheet
its said that appropriate application would be SDS-page but are these
proteins also usable in for example indirect ELISA when used as an
antigen to be recognized with antibody?
Thank you!

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Answer

Thank you for contacting us and for your interest in our products.

I am sorry to confirm that as far as we are aware, ab127252 has never been tested in ELISA. All tested and guaranteed applications are specified on our datasheets, and these are updated as soon as any new information is brought to our attention.

In this case, I can sugggest to take into consideration that this peptide contains only amino acids 43-210 of the protein. So you would need to ensure that the antibody usedwill detect this part of the protein by checking the immunogen sequence.

The protein is also tagged, which could sometimes interfere with antibody binding.

If you do decide to try the protien in ELISA, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact me for any further advice or information.

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