Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 80% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant human IDH1 protein
See all IDH1 proteins and peptides
Specific Activity: 5600 pmol/min/µg.
Assay Conditions: Reaction was conducted in 50 mM Tris (pH 7.4), 10 mM MgCl2, 200 µM DL-isocitrate, and 200 µM NADP. Incubate for 30 minutes at room temperature. Read at Abs340.
Purity> 80 % SDS-PAGE.
Expression systemBaculovirus infected Sf9 cells
Protein lengthFull length protein
SequenceMSKKISGGSVVEMQGDEMTRIIWELIKEKLIFPYVELDLHSYDLGIENRD ATNDQVTKDAAEAIKKHNVGVKCATITPDEKRVEEFKLKQMWKSPNGTIR NILGGTVFREAIICKNIPRLVSGWVKPIIIGRHAYGDQYRATDFVVPGPG KVEITYTPSDGTQKVTYLVHNFEEGGGVAMGMYNQDKSIEDFAHSSFQMA LSKGWPLYLSTKNTILKKYDGRFKDIFQEIYDKQYKSQFEAQKIWYEHRL IDDMVAQAMKSEGGFIWACKNYDGDVQSDSVAQGYGSLGMMTSVLVCPDG KTVEAEAAHGTVTRHYRMYQKGQETSTNPIASIFAWTRGLAHRAKLDNNK ELAFFANALEEVSIETIEAGFMTKDLAACIKGLPNVQRSDYLNTFEFMDK LGENLKIKLAQAKL
Predicted molecular weight48 kDa including tags
Amino acids1 to 414
TagsDDDDK tag C-Terminus
Additional sequence informationGenBank Accession No. NM_005896.
Our Abpromise guarantee covers the use of ab198096 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product was previously labelled as Isocitrate dehydrogenase
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.71% Tris HCl, 0.72% Sodium chloride, 0.02% Potassium chloride, 0.05% DTT, 10% Glycerol
Contains 90 µg/ml DDDDK peptide.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Cytosolic NADP isocitrate dehydrogenase
- Cytosolic NADP-isocitrate dehydrogenase
- Epididymis luminal protein 216
Involvement in diseaseGlioma
Genetic variations are associated with cartilaginous tumors such as enchondroma or chondrosarcoma. Mutations of Arg-132 to Cys, Gly or His abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R(-)-2-hydroxyglutarate.
Sequence similaritiesBelongs to the isocitrate and isopropylmalate dehydrogenases family.
modificationsAcetylation at Lys-374 dramatically reduces catalytic activity.
Cellular localizationCytoplasm. Peroxisome.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab198096 has not yet been referenced specifically in any publications.