Overview

Description

  • Nature

    Recombinant
  • Source

    Wheat germ
  • Amino Acid Sequence
    • Species

      Human
    • Sequence

      MLCYVTRPDAVLMEVEVEAKANGEDCLNQVCRRLGIIEVDYFGLQFTGSK GESLWLNLRNRISQQMDGLAPYRLKLRVKFFVEPHLILQEQTRHIFFLHI KEALLAGHLLCSPEQAVELSALLAQTKFGDYNQNTAKYNYEELCAKELSS ATLNSIVAKHKELEGTSQASAEYQVLQIVSAMENYGIEWHSVRDSEGQKL LIGVGPEGISICKDDFSPINRIAYPVVQMATQSGKNVYLTVTKESGNSIV LLFKMISTRAASGLYRAITETHAFYRCDTVTSAVMMQYSRDLKGHLASLF LNENINLGKKYVFDIKRTSKEVYDHARRALYNAGVVDLVSRNNQSPSHSP LKSSESSMNCSSCEGLSCQQTRVLQEKLRKLKEAMLCMVCCEEEINSTFC PCGHTVCCESCAAQLQSCPVCRSRVEHVQHVYLPTHTSLLNLTVI
    • Amino acids

      1 to 445
    • Tags

      GST tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab153303 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    ELISA

  • Form

    Liquid
  • Additional notes

    Protein concentration is above or equal to 0.05 mg/ml.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.31% Glutathione, 0.79% Tris HCl

General Info

  • Alternative names

    • BM-023
    • BZF1
    • E3 ubiquitin protein ligase MYLIP
    • E3 ubiquitin-protein ligase MYLIP
    • Idol
    • Inducible degrader of the LDL-receptor
    • Lipoprotein receptor
    • MIR
    • MYLIP
    • MYLIP_HUMAN
    • Myosin regulatory light chain interacting protein
    • PP5242
    see all
  • Function

    E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Proteasomal degradation of MRLC leads to inhibit neurite outgrowth in presence of NGF by counteracting the stabilization of MRLC by saposin-like protein (CNPY2/MSAP) and reducing CNPY2-stimulated neurite outgrowth. Acts as a sterol-dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR.
  • Tissue specificity

    Ubiquitously expressed.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Contains 1 FERM domain.
    Contains 1 RING-type zinc finger.
  • Developmental stage

    Expressed in fetal tissues and higher levels were detected in placenta and fetal lung.
  • Domain

    The RING domain mediates ubiquitination and the neurite outgrowth inhibitory activity.
  • Post-translational
    modifications

    Autoubiquitinated.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt

Images

  • ab153303 on a 12.5% SDS-PAGE stained with Coomassie Blue.

References

ab153303 has not yet been referenced specifically in any publications.

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