Recombinant Human IDOL protein (ab198638)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 89% SDS-PAGE
- Tags: His-DDDDK tag N-Terminus
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant Human IDOL protein -
Purity
>= 89 % SDS-PAGE.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
-
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Sequence
MHHHHHHDYKDDDDKLCYVTRPDAVLMEVEVEAKANGEDCLNQVCRRLGI IEVDYFGLQFTGSKGESLWLNLRNRISQQMDGLAPYRLKLRVKFFVEPHL ILQEQTRHIFFLHIKEALLAGHLLCSPEQAVELSALLAQTKFGDYNQNTA KYNYEELCAKELSSATLNSIVAKHKELEGTSQASAEYQVLQIVSAMENYG IEWHSVRDSEGQKLLIGVGPEGISICKDDFSPINRIAYPVVQMATQSGKN VYLTVTKESGNSIVLLFKMISTRAASGLYRAITETHAFYRCDTVTSAVMM QYSRDLKGHLASLFLNENINLGKKYVFDIKRTSKEVYDHARRALYNAGVV DLVSRNNQSPSHSPLKSSESSMNCSSCEGLSCQQTRVLQEKLRKLKEAML CMVCCEEEINSTFCPCGHTVCCESCAAQLQSCPVCRSRVEHVQHVYLPTH TSLLNLTVI -
Predicted molecular weight
16 kDa including tags -
Amino acids
2 to 445 -
Tags
His-DDDDK tag N-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab198638 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.017% Potassium chloride, 20% Glycerol (glycerin, glycerine)
Contains 80 µg/ml DDDDK peptide
General Info
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Alternative names
- BM-023
- BZF1
- E3 ubiquitin protein ligase MYLIP
see all -
Function
E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Proteasomal degradation of MRLC leads to inhibit neurite outgrowth in presence of NGF by counteracting the stabilization of MRLC by saposin-like protein (CNPY2/MSAP) and reducing CNPY2-stimulated neurite outgrowth. Acts as a sterol-dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR. -
Tissue specificity
Ubiquitously expressed. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 FERM domain.
Contains 1 RING-type zinc finger. -
Developmental stage
Expressed in fetal tissues and higher levels were detected in placenta and fetal lung. -
Domain
The RING domain mediates ubiquitination and the neurite outgrowth inhibitory activity. -
Post-translational
modificationsAutoubiquitinated. -
Cellular localization
Cytoplasm. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab198638 has been referenced in 1 publication.
- Chan ML et al. Effects of statins on the inducible degrader of low-density lipoprotein receptor in familial hypercholesterolemia. Endocr Connect 11:N/A (2022). PubMed: 35560019