Recombinant Human IFT20 protein (ab134549)
- Datasheet
- References
- Protocols
Description
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Product name
Recombinant Human IFT20 protein -
Purity
> 95 % SDS-PAGE.
ab134549 is purified using conventional chromatography techniques. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MGSSHHHHHHSSGLVPRGSHMAKDILGEAGLHFDELNKLRVLDPEVTQQT IELKEECKDFVDKIGQFQKIVGGLIELVDQLAKEAENEKMKAIGARNLLK SIAKQREAQQQQLQALIAEKKMQLERYRVEYEALCKVEAEQNEFIDQFIF QK -
Predicted molecular weight
17 kDa including tags -
Amino acids
1 to 132 -
Tags
His tag N-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab134549 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Mass Spectrometry
SDS-PAGE
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Mass spectrometry
MALDI-TOF -
Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.02% DTT, 0.32% Tris HCl, 30% Glycerol, 0.88% Sodium chloride
General Info
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Alternative names
- hIFT20
- Intraflagellar transport 20 homolog (Chlamydomonas)
- Intraflagellar transport protein 20 like
- Intraflagellar transport protein IFT20
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Relevance
Eukaryotic cilia are assembled via intraflagellar transport (IFT) in which large protein particles are motored along ciliary microtubules. The IFT20 subunit of a particle is localized to the Golgi complex, plus the basal body and cilia. In living cells, fluorescently tagged IFT20 is highly dynamic and moves between the Golgi complex and the cilium as well as along ciliary microtubules. It has been suggested that IFT20 functions in the delivery of ciliary membrane proteins from the Golgi complex to the cilium. There are three different isoforms. -
Cellular localization
Golgi apparatus. Cilium. Centrosome, centriole.
Images
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SDS-PAGE - Recombinant Human IFT20 protein (ab134549)15% SDS-PAGE analysis of 3 µg ab134549.
Datasheets and documents
References
ab134549 has not yet been referenced specifically in any publications.
