Recombinant Human IFT20 protein (ab134549)

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SDS-PAGE - Recombinant Human IFT20 protein (ab134549)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 95% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: MS, SDS-PAGE

    Description

    • Product name

      Recombinant Human IFT20 protein

    • Purity

      > 95 % SDS-PAGE.
      ab134549 is purified using conventional chromatography techniques.

    • Expression system

      Escherichia coli

    • Accession

      Q8IY31

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MGSSHHHHHHSSGLVPRGSHMAKDILGEAGLHFDELNKLRVLDPEVTQQT IELKEECKDFVDKIGQFQKIVGGLIELVDQLAKEAENEKMKAIGARNLLK SIAKQREAQQQQLQALIAEKKMQLERYRVEYEALCKVEAEQNEFIDQFIF QK

      • Predicted molecular weight

        17 kDa including tags

      • Amino acids

        1 to 132

      • Tags

        His tag N-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab134549 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Mass Spectrometry

      SDS-PAGE

    • Mass spectrometry

      MALDI-TOF

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 0.02% DTT, 0.32% Tris HCl, 30% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

    General Info

    • Alternative names

      • hIFT20
      • Intraflagellar transport 20 homolog (Chlamydomonas)
      • Intraflagellar transport protein 20 like
      • Intraflagellar transport protein IFT20

    • Relevance

      Eukaryotic cilia are assembled via intraflagellar transport (IFT) in which large protein particles are motored along ciliary microtubules. The IFT20 subunit of a particle is localized to the Golgi complex, plus the basal body and cilia. In living cells, fluorescently tagged IFT20 is highly dynamic and moves between the Golgi complex and the cilium as well as along ciliary microtubules. It has been suggested that IFT20 functions in the delivery of ciliary membrane proteins from the Golgi complex to the cilium. There are three different isoforms.

    • Cellular localization

      Golgi apparatus. Cilium. Centrosome, centriole.

    Images

    • SDS-PAGE - Recombinant Human IFT20 protein (ab134549)
      SDS-PAGE - Recombinant Human IFT20 protein (ab134549)
      15% SDS-PAGE analysis of 3 µg ab134549.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab134549? Please let us know so that we can cite the reference in this datasheet.

    ab134549 has not yet been referenced specifically in any publications.

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