Description

  • Product name

    Recombinant human IGF1 Receptor beta subunit protein
  • Biological activity

    Specific activity: ~100 nmol/min/mg at 25°C using poly(Glu:Tyr)4:1 substrate. Reaction conditions are 50 mM Tris, pH 7.5, 5 mM MgCl2, 5 mM MnCl2, 1 mM DTT, 250 µM ATP. The kinase concentration was 0.5-1 µM and poly-L-lysine was added in equimolar amounts to the kinase.
  • Expression system

    Insect cells
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Amino acids

      930 to 1337

Specifications

Our Abpromise guarantee covers the use of ab80349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 7.50
    Constituents: 0.0154% DTT, 0.605% Tris

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • CD221
    • CD221 antigen
    • EC 2.7.10.1
    • IGF I receptor
    • IGF-I receptor
    • Igf1r
    • IGF1R_HUMAN
    • IGFIR
    • Insulin like growth factor 1 receptor alpha chain
    • Insulin like growth factor 1 receptor beta chain
    • Insulin like growth factor I receptor
    • Insulin-like growth factor 1 receptor beta chain
    • Insulin-like growth factor I receptor
    • JTK13
    • MGC142170
    • MGC142172
    • MGC18216
    • Soluble IGF1R variant 1
    • Soluble IGF1R variant 2
    see all
  • Function

    This receptor binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. It has a tyrosine-protein kinase activity, which is necessary for the activation of the IGF1-stimulated downstream signaling cascade. When present in a hybrid receptor with INSR, binds IGF1. PubMed:12138094 shows that hybrid receptors composed of IGF1R and INSR isoform Long are activated with a high affinity by IGF1, with low affinity by IGF2 and not significantly activated by insulin, and that hybrid receptors composed of IGF1R and INSR isoform Short are activated by IGF1, IGF2 and insulin. In contrast, PubMed:16831875 shows that hybrid receptors composed of IGF1R and INSR isoform Long and hybrid receptors composed of IGF1R and INSR isoform Short have similar binding characteristics, both bind IGF1 and have a low affinity for insulin.
  • Tissue specificity

    Found as a hybrid receptor with INSR in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibroblasts, spleen and placenta (at protein level). Expressed in a variety of tissues.
  • Involvement in disease

    Defects in IGF1R are a cause of insulin-like growth factor 1 resistance (IGF1RES) [MIM:270450]. It is a disorder characterized by intrauterine growth retardation and poor postnatal growth accompanied with increased plasma IGF1.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
    Contains 3 fibronectin type-III domains.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    The cytoplasmic domain of the beta subunit is autophosphorylated on tyrosine residues in response to insulin-like growth factor I (IGF I).
    Phosphorylation of Tyr-980 is required for IRS1- and SHC1-binding.
  • Cellular localization

    Membrane.
  • Information by UniProt

References

ab80349 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for contacting us.

This productdoesn’t contain p-IGF-1R beta and doesn’t need to phosphorylate it in vitro for the enzyme activity.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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