Description

  • Product name

    Recombinant human IGF2 protein (Active)
    See all IGF2 proteins and peptides
  • Biological activity

    FDC-P1 cell proliferation. ≤15 ng/mL; ≥ 6.7 x 10^4 units/mg (typical ED50 is 1-10 ng/mL).

  • Purity

    > 95 % SDS-PAGE.

  • Endotoxin level

    < 1.000 Eu/µg
  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      AYRPSETLCGGELVDTLQFVCGDRGFYFSRPASRVSRRSRGIVEECCFRS CDLALLETYCATPAKSE
    • Predicted molecular weight

      8 kDa
    • Amino acids

      25 to 91
    • Additional sequence information

      Full length mature chain without signal peptide, without propeptide.

Specifications

Our Abpromise guarantee covers the use of ab256027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at room temperature. Store at -20°C.

    Constituent: 0.1% Trifluoroacetic acid

    0.2 micron filtered

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute in sterile water at 0.1 mg/ml. Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

General Info

  • Alternative names

    • C11orf43
    • IGF 2
    • IGF II
    • IGF-II
    • IGF2
    • IGF2_HUMAN
    • IGFII
    • INSIGF
    • Insulin like Growth Factor 2
    • insulin like growth factor 2 (somatomedin A)
    • Insulin like growth factor II
    • Insulin like growth factor II precursor
    • Insulin like growth factor type 2
    • pp9974
    • Preptin
    • putative insulin like growth factor II associated protein
    • Somatomedin A
    • Somatomedin-A
    see all
  • Function

    The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.
    Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.
  • Involvement in disease

    Epigenetic changes of DNA hypomethylation in IGF2 are a cause of Silver-Russell syndrome (SIRS) [MIM:180860]. SIRS is a clinically heterogeneous condition characterized by severe intrauterine growth retardation, poor postnatal growth, craniofacial features such as a triangular shaped face and a broad forehead, body asymmetry, and a variety of minor malformations.
  • Sequence similarities

    Belongs to the insulin family.
  • Post-translational
    modifications

    O-glycosylated with a core 1 or possibly core 8 glycan.
  • Cellular localization

    Secreted.
  • Information by UniProt

Images

  • FDC-P1 cell proliferation. ≤15 ng/mL; ≥ 6.7 x 10^4 units/mg (typical ED50 is 1-10 ng/mL).

  • SDS-PAGE analysis of ab256027 (1μg) under non-reducing (Lane 1) and reducing (Lane 2) conditions.

    Human IGF2 is predicted to have a MW of 7.5kDa.
    4-20% Tris-Glycine gel. Coomassie Blue staining.

References

ab256027 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab256027.
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