Description

  • Product name

    Recombinant human IGFBP1 protein
    See all IGFBP1 proteins and peptides
  • Biological activity

    Biological Activity : The ED50, as determined by its ability to inhibit IGF-I induced proliferation of MCF-7, is < 0.5 µg/ml in the presence of 6 ng/ml of human IGF-I.
  • Endotoxin level

    < 0.100 Eu/µg
  • Expression system

    Escherichia coli
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MAPWQCAPCS AEKLALCPPV SASCSEVTRS AGCGCCPMCA LPLGAACGVA TARCARGLSC RALPGEQQPL HALTRGQGAC VQESDASAPH AAEAGSPESP ESTEITEEEL LDNFHLMAPS EEDHSILWDA ISTYDGSKAL HVTNIKKWKE PCRIELYRVV ESLAKAQETS GEEISKFYLP NCNKNGFYHS RQCETSMDGE AGLCWCVYPW NGKRIPGSPE IRGDPNCQIY FNVQN

Associated products

Specifications

Our Abpromise guarantee covers the use of ab50216 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

    Functional Studies

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C long term.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconsitute to 1mg/ml with sterile water.

General Info

  • Alternative names

    • AFBP
    • Alpha pregnancy associated endometrial globulin
    • Amniotic fluid binding protein
    • Binding protein 25
    • Binding protein 26
    • Binding protein 28
    • Growth hormone independent binding protein
    • hIGFBP 1
    • hIGFBP1
    • IBP 1
    • IBP-1
    • IBP1
    • IBP1_HUMAN
    • IGF binding protein 1
    • IGF BP25
    • IGF-binding protein 1
    • IGFBP 1
    • IGFBP-1
    • IGFBP1
    • Insulin like growth factor binding protein 1
    • Insulin-like growth factor-binding protein 1
    • Placental protein 12
    • PP 12
    • PP12
    see all
  • Function

    IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration.
  • Sequence similarities

    Contains 1 IGFBP N-terminal domain.
    Contains 1 thyroglobulin type-1 domain.
  • Post-translational
    modifications

    Phosphorylated; probably by casein kinase II. Alters the affinity of the protein for IGFs.
  • Cellular localization

    Secreted.
  • Information by UniProt

Images

  • Anti-IGFBP1 antibody [33627.11] (ab94985) at 1 µg/ml + Recombinant human IGFBP1 protein (ab50216) at 0.1 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 30 seconds

References

ab50216 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab50216.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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