Recombinant human IGFBP2 protein (ab63223)
Key features and details
- Expression system: Insect cells
- Purity: > 98% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human IGFBP2 protein -
Biological activity
Biological Activity : Determined by its ability to inhibit IGF-I induced proliferation of FDC-P1 cells.
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Purity
> 98 % SDS-PAGE.
Greater than 98% by SDS-PAGE gel and HPLC analyses. Endotoxin level is < 0.1 ng/µg of protein (<1EU/µg). -
Endotoxin level
< 1.000 Eu/µg -
Expression system
Insect cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
EVLFRCPPCT PERLAACGPP PVAPPAAVAA VAGGARMPCA ELVREPGCGC CSVCARLEGE ACQVYTPRCG QGLRCYPHPG SELPLQALVM GEGTCEKRRD AEYGASPEQV ADNGDDHSEG GLVENHVDST MNMLGGGGSA GRKPLKSGMK ELAVFREKVT EQHRQMGKGG KHHLGLEEPK KLRPPPARTP CQQELDQVLE RISTMRLPDE RGPLEHLYSL HIPNCDKHGL YNLKQCKMSL NGQRGECWCV NPNTGKLIQG APTIRGDPEC HLFYNEQQEA RGVHTQRMQ -
Predicted molecular weight
32 kDa -
Amino acids
37 to 325 -
Additional sequence information
The sequence is the full length mature form minus the signal peptide.
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Specifications
Our Abpromise guarantee covers the use of ab63223 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
Functional Studies
SDS-PAGE
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionFor lot specific reconstitution information please contact our Scientific Support Team.
General Info
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Alternative names
- BP 2
- BP2
- IBP 2
see all -
Function
Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. -
Sequence similarities
Contains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain. -
Domain
The C-terminus is required for IGF-binding and growth inhibition. -
Post-translational
modificationsO-glycosylated. -
Cellular localization
Secreted. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (4)
ab63223 has been referenced in 4 publications.
- Tan X et al. A protumorigenic secretory pathway activated by p53 deficiency in lung adenocarcinoma. J Clin Invest 131:N/A (2021). PubMed: 32931483
- Shimizu M et al. Metallothionein 2A Expression in Cancer-Associated Fibroblasts and Cancer Cells Promotes Esophageal Squamous Cell Carcinoma Progression. Cancers (Basel) 13:N/A (2021). PubMed: 34572779
- Li T et al. IGFBP2 regulates PD-L1 expression by activating the EGFR-STAT3 signaling pathway in malignant melanoma. Cancer Lett 477:19-30 (2020). PubMed: 32120023
- Chua CY et al. IGFBP2 potentiates nuclear EGFR-STAT3 signaling. Oncogene 35:738-47 (2016). PubMed: 25893308