Product nameRecombinant Human IKB alpha protein
See all IKB alpha proteins and peptides
Protein lengthFull length protein
Amino Acid Sequence
SequenceMGSSHHHHHHSSGLVPRGSHMFQAAERPQEWAMEGPRDGLKKERLLDDRH DSGLDSMKDEEYEQMVKE LQEIRLEPQEVPRGSEPWKQQLTEDGDSFL HLAIIHEEKALTMEVIRQVKGDLAFLNFQNNLQQTPLHLAVITNQ PEI AEALLGAGCDPELRDFRGNTPLHLACEQGCLASVGVLTQSCTTPHLHSIL KATNYNGHTCLHLASIHGYLGI VELLVSLGADVNAQEPCNGRTALHLA VDLQNPDLVSLLLKCGADVNRVTYQGYSPYQLTWGRPSTRIQQQLGQLT LENLQMLPESEDEESYDTESEFTEFTEDELPYDDCVFGGQRLTL
Molecular weight38 kDa including tags
Amino acids1 to 317
TagsHis tag N-Terminus
- Anti-IKB alpha antibody [6A920] (ab12134)
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-IKB alpha antibody [E130] (ab32518)
- Anti-6X His tag® antibody [4D11] (ab5000)
- Anti-IKB alpha antibody [7A4] (ab72135)
- Anti-IKB alpha antibody [EP697] (ab76429)
- Anti-IKB alpha antibody (ab95338)
- Anti-IKB alpha antibody (ab97783)
Our Abpromise guarantee covers the use of ab113133 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Purity> 90 % SDS-PAGE.
ab113133 was purified by using conventional chromatography techniques.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: 0.32% Tris HCl, 0.02% DTT, 20% Glycerol, 0.58% Sodium chloride
- I kappa B alpha
FunctionInhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
Involvement in diseaseEctodermal dysplasia, anhidrotic, with T-cell immunodeficiency autosomal dominant
Sequence similaritiesBelongs to the NF-kappa-B inhibitor family.
Contains 5 ANK repeats.
modificationsPhosphorylated; disables inhibition of NF-kappa-B DNA-binding activity. Phosphorylation at positions 32 and 36 is prerequisite to recognition by UBE2D3 leading to polyubiquitination and subsequent degradation.
Sumoylated; sumoylation requires the presence of the nuclear import signal. Sumoylation blocks ubiquitination and proteasome-mediated degradation of the protein thereby increasing the protein stability.
Monoubiquitinated at Lys-21 and/or Lys-22 by UBE2D3. Ubiquitin chain elongation is then performed by CDC34 in cooperation with the SCF(FBXW11) E3 ligase complex, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. The resulting polyubiquitination leads to protein degradation. Also ubiquitinated by SCF(BTRC) following stimulus-dependent phosphorylation at Ser-32 and Ser-36.
Deubiquitinated by porcine reproductive and respiratory syndrome virus Nsp2 protein, which thereby interferes with NFKBIA degradation and impairs subsequent NF-kappa-B activation.
Cellular localizationCytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export.
- Information by UniProt
ab113133 has not yet been referenced specifically in any publications.