Recombinant human IKK alpha protein (Active) (ab102103)

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Functional Studies - Recombinant human IKK alpha protein (ab102103)
  • SDS-PAGE - Recombinant human IKK alpha protein (ab102103)
  • SDS-PAGE - Recombinant human IKK alpha protein (ab102103)
  • SDS-PAGE - Recombinant human IKK alpha protein (ab102103)
  • Functional Studies - Recombinant human IKK alpha protein (ab102103)
  • Western blot - Recombinant human IKK alpha protein (ab102103)

Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Purity: > 75% Densitometry
  • Active: Yes
  • Tags: GST tag N-Terminus
  • Suitable for: SDS-PAGE, WB, Functional Studies

Description

  • Product name

    Recombinant human IKK alpha protein (Active)

  • Biological activity

    Specific activity is 2.5nm/min/mg

  • Purity

    > 75 % Densitometry.
    ab102103 was determined to be >75% pure by densitometry.

  • Expression system

    Baculovirus infected Sf9 cells

  • Accession

    O15111

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Molecular weight information

      Approx 114 kDa by SDS-PAGE

    • Amino acids

      1 to 745

    • Tags

      GST tag N-Terminus

    • Additional sequence information

      GenBank: BC092514

Associated products

Specifications

Our Abpromise guarantee covers the use of ab102103 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Western blot

    Functional Studies

  • Form

    Liquid

  • Additional notes

    ab204863 (IKB alpha peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.307% Glutathione, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • chuk
    • CHUK1
    • Conserved Helix Loop Helix Ubiquitous Kinase
    • Conserved helix loop ubiquitous kinase
    • Conserved helix-loop-helix ubiquitous kinase
    • I Kappa B Kinase 1
    • I Kappa B Kinase Alpha
    • I-kappa-B kinase 1
    • I-kappa-B kinase alpha
    • IkappaB kinase
    • IkB kinase alpha subunit
    • IkBKA
    • IKK 1
    • IKK A
    • IKK a kinase
    • IKK-A
    • IKK-alpha
    • IKK1
    • IKKA
    • IKKA_HUMAN
    • Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B Cells
    • Inhibitor Of Nuclear Factor Kappa B Kinase Alpha Subunit
    • Inhibitor of nuclear factor kappa-B kinase subunit alpha
    • NFKBIKA
    • Nuclear Factor Kappa B Inhibitor Kinase Alpha
    • Nuclear factor NF kappa B inhibitor kinase alpha
    • Nuclear factor NF-kappa-B inhibitor kinase alpha
    • Nuclear factor NFkappaB inhibitor kinase alpha
    • Nuclear Factor Of Kappa Light Chain Gene Enhancer In B Cells Inhibitor
    • TCF-16
    • TCF16
    • Transcription factor 16
    see all

  • Function

    Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines.

  • Tissue specificity

    Widely expressed.

  • Involvement in disease

    Defects in CHUK are the cause of cocoon syndrome (COCOS) [MIM:613630]; also known as fetal encasement syndrome. COCOS is a lethal syndrome characterized by multiple fetal malformations including defective face and seemingly absent limbs, which are bound to the trunk and encased under the skin.

  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
    Contains 1 protein kinase domain.

  • Post-translational
    modifications

    Phosphorylated by MAP3K14/NIK, AKT and to a lesser extent by MEKK1, and dephosphorylated by PP2A. Autophosphorylated.
    Acetylation of Thr-179 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B signaling pathway.

  • Cellular localization

    Cytoplasm. Nucleus. Shuttles between the cytoplasm and the nucleus.
  • Information by UniProt

Images

  • Functional Studies - Recombinant human IKK alpha protein (ab102103)
    Functional Studies - Recombinant human IKK alpha protein (ab102103)
    The specific activity of IKK alpha (ab102103) was determined to be 2.9 nmol/min/mg as per activity assay protocol
  • SDS-PAGE - Recombinant human IKK alpha protein (ab102103)
    SDS-PAGE - Recombinant human IKK alpha protein (ab102103)
    SDS PAGE analysis of ab102103
  • SDS-PAGE - Recombinant human IKK alpha protein (ab102103)
    SDS-PAGE - Recombinant human IKK alpha protein (ab102103)
    SDS PAGE analysis of ab102103
  • SDS-PAGE - Recombinant human IKK alpha protein (ab102103)
    SDS-PAGE - Recombinant human IKK alpha protein (ab102103)
    ab102103 was determined to be >75% pure by densitometry. MWt ~114 kDa.
  • Functional Studies - Recombinant human IKK alpha protein (ab102103)
    Functional Studies - Recombinant human IKK alpha protein (ab102103)
    The specific activity of ab102103 was determined to be 2.5 nmol/mg/min.
  • Western blot - Recombinant human IKK alpha protein (ab102103)
    Western blot - Recombinant human IKK alpha protein (ab102103)
    Anti-IKK alpha antibody [Y463] (ab32041) at 1/10000 dilution + Recombinant human IKK alpha protein (Active) (ab102103) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 10 seconds

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab102103? Please let us know so that we can cite the reference in this datasheet.

ab102103 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Question

Dear technical team, Our cusotmer would like to purchase ab102103. He is going to treat ab102103 to cells but the tested applications are FuncS, WB. Is it possible to use this purpose or request discount code for it? Check this for our customer. Thank you. Best regards,            

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Answer

Thank you for your enquiry. I can confirm we can provide our discount program for previously untested species and appilcations, and also where there is no image available on the datasheet for an application that is already tested.  In this case, for the ab102103  IKK alpha protein (Active), this has already been tested in WB and in functional assays, and we already have data on the datasheet. I am sorry this therefore does not qualify for a testing discount on this occasion. I would like to reassure the customer that the tested applications listed on the datasheet, in this case WB and Functional studies, will be covered by our Abpomise guarantee should they encounter any difficulties.  The guarantee period is 6 months after purchase. We would still like to encourage the customer to send an Abreview, for which they would obtain Abpoints. We always encourage customers to send their results back to us, whether positive or negative, and we make all product information available to researchers. To find out more about our Abreview system, please see the following webpage: https://www.abcam.com/abreviews   I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.  

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