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  1. Link

    recombinant-human-il-10-protein-active-ab259402.pdf

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Recombinant human IL-10 protein (Active) (ab259402)

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Functional Studies - Recombinant human IL-10 protein (Active) (ab259402)
  • SDS-PAGE - Recombinant human IL-10 protein (Active) (ab259402)
  • HPLC - Recombinant human IL-10 protein (Active) (ab259402)
  • Mass Spectrometry - Recombinant human IL-10 protein (Active) (ab259402)

Key features and details

  • Expression system: HEK 293 cells
  • Purity: >= 95% SDS-PAGE
  • Endotoxin level: <= 0.005 Eu/µg
  • Active: Yes
  • Suitable for: Functional Studies, MS, HPLC, SDS-PAGE, Cell Culture

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Description

  • Product name

    Recombinant human IL-10 protein (Active)
    See all IL-10 proteins and peptides
  • Biological activity

    Fully biologically active, as determined by dose dependant stimulation of human MC/9 cells.

    ED50 is ≤ 3 ng/ mL , corresponding to a specific activity of 3.33 x 105 units/mg.

  • Purity

    >= 95 % SDS-PAGE.
    Purity by HPLC >=95%.
  • Endotoxin level

    <=0.005 Eu/µg
  • Expression system

    HEK 293 cells
  • Accession

    P22301
  • Protein length

    Protein fragment
  • Animal free

    Yes
  • Carrier free

    Yes
  • Nature

    Recombinant
    • Species

      Human
    • Predicted molecular weight

      18 kDa
    • Molecular weight information

      M+0.17 Da (Calc. mass 17991.83 Da). SENSCTHFPGNLPNMLRDLRDAFSRVKTFFQMKDQLDNLLLKESLLEDFKGYLGCQALSEMIQFYLEEVMPQAENQDPDIKAHVNSLGENLKTLRLRLRRCHRFLPCENKSKAVEQVKNAFNKLQEKGIYKAMSEFDIFINYIEAYMTMKIRN
    • Amino acids

      26 to 178

Specifications

Our Abpromise guarantee covers the use of ab259402 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    Mass Spectrometry

    HPLC

    SDS-PAGE

    Cell Culture

  • Form

    Lyophilized
  • Additional notes

    This product will no longer be available once inventory is depleted. It is being replaced by ab284660. This protein has an improved amino acid sequence, 19-178, matching the mature full length IL-10 protein.

    This protein is filter sterilised prior to aliquoting and lyophilisation. All aliquoting and lyophilisation steps are performed in a sterile environment.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at Room Temperature. Store at Room Temperature.

    Information available upon request.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute with phosphate buffered saline.Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1 week.Avoid repeated freeze-thaw. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.

General Info

  • Alternative names

    • CSIF
    • Cytokine synthesis inhibitory factor
    • GVHDS
    • IL 10
    • IL-10
    • IL10
    • IL10_HUMAN
    • IL10A
    • Interleukin 10
    • Interleukin-10
    • MGC126450
    • MGC126451
    • T-cell growth inhibitory factor
    • TGIF
    see all
  • Function

    Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
  • Tissue specificity

    Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
  • Sequence similarities

    Belongs to the IL-10 family.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P22301 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Images

  • Functional Studies - Recombinant human IL-10 protein (Active) (ab259402)
    Functional Studies - Recombinant human IL-10 protein (Active) (ab259402)

    Fully biologically active, as determined by dose dependant stimulation of human MC/9 cells.

    ED50 is ≤ 3 ng/ mL , corresponding to a specific activity of 3.33 x 105 units/mg.

  • SDS-PAGE - Recombinant human IL-10 protein (Active) (ab259402)
    SDS-PAGE - Recombinant human IL-10 protein (Active) (ab259402)

    SDS-PAGE analysis of ab259402.

  • HPLC - Recombinant human IL-10 protein (Active) (ab259402)
    HPLC - Recombinant human IL-10 protein (Active) (ab259402)

    Purity = 97%.

    The spectrum was recorded using a 1260 Infinity II HPLC system with DAD and a MabPac RP column (3.0x100 mm, 4 µm). 5 µL of purified protein was injected and the gradient run from 80 % water:TFA (99.9:0.1 v/v) and 20 % acetonitrile:water:TFA (90:9.9:0.1 v/v/v) to 20 % water:TFA (99.9:0.1 v/v) and 80 % acetonitrile:water:TFA (90:9.9:0.1 v/v/v) within 3 minutes followed by an isocratic step for another 3 min. Flow rate was 0.5 mL/min and the column compartment temperature was 50°C.

  • Mass Spectrometry - Recombinant human IL-10 protein (Active) (ab259402)
    Mass Spectrometry - Recombinant human IL-10 protein (Active) (ab259402)

    M+0.17 Da (Calc. mass 17991.83 Da).

    The spectrum was recorded with a 6545XT AdvanceBio LC/Q-TOF (Agilent Technologies) and a MabPac RP column (42.1x50 mm, 4 µm, Thermo Scientific). 5 µL of purified protein was injected and the gradient run from 85 % water:FA (99.9:0.1 v/v) and 15 % acetonitrile:FA (90:9.9:0.1 v/v/v) to 55 % water:FA (99.9:0.1 v/v) and 45 % acetonitrile:FA (90:9.9:0.1 v/v/v) within 3 minutes followed by an isocratic step for another 2.5 min. Flow rate was 0.4 mL/min and the column compartment temperature was 60°C. Data was analysed and deconvoluted using the Bioconfirm software (Agilent Technologies).

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab259402? Please let us know so that we can cite the reference in this datasheet.

ab259402 has not yet been referenced specifically in any publications.

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