Key features and details
- Expression system: Escherichia coli
- Purity: > 98% SDS-PAGE
- Active: Yes
- Suitable for: HPLC, Functional Studies, SDS-PAGE
- High batch-to-batch consistency
- Optimal bioactivity
- Guaranteed identical to human native proteins
- >95% purity
- Ultra-low endotoxin levels: <0.005 Eu/µg
- Carrier and tag free
Product nameRecombinant human IL-10 protein (Animal Free)
See all IL-10 proteins and peptides
Determined by the dose-dependent co-stimulation (with Human IL-4) of MC/9 cells. The expected ED50 is ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5 x 105 units/mg.
Purity> 98 % SDS-PAGE.
> 98% by HPLC analysis.
Expression systemEscherichia coli
Protein lengthFull length protein
SequenceMSPGQGTQSENSCTHFPGNLPNMLRDLRDAFSRVKTFFQMKDQLDNLLLK ESLLEDFKGYLGCQALSEMIQFYLEEVMPQAENQDPDIKAHVNSLGENLK TLRLRLRRCHRFLPCENKSKAVEQVKNAFNKLQEKGIYKAMSEFDIFINY IEAYMTMKIRN
Predicted molecular weight19 kDa
Amino acids19 to 178
Additional sequence informationThis product is for the mature full length protein. The signal peptide is not included.
Our Abpromise guarantee covers the use of ab217418 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionFor lot specific reconstitution information please contact our Scientific Support Team.
- Cytokine synthesis inhibitory factor
FunctionInhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
Tissue specificityProduced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
Sequence similaritiesBelongs to the IL-10 family.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab217418 has not yet been referenced specifically in any publications.