Key features and details
- Expression system: Baculovirus infected insect cells
- Purity: > 90% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE
Product nameRecombinant human IL-12 protein (His tag)
See all IL-12 proteins and peptides
The activity is determined by the IFN-g ELISA in a using NK-92 cell. The ED50 for this effect is less than or equal to 0.03 ng/ml.
Purity> 90 % SDS-PAGE.
Endotoxin level< 1.000 Eu/µg
Expression systemBaculovirus infected insect cells
Protein lengthFull length protein
Predicted molecular weight58 kDa including tags
TagsHis tag C-Terminus
Our Abpromise guarantee covers the use of ab187480 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab187480 is a heterodimer of IL-12A (amino acids 23 - 219; UniProt P29459) and IL-12B (amino acids 23 - 328; UniProt P29460) linked through a disulfide-bond between cysteines. The molecular weight of IL-12A (p35) is 23.3 kDa (203aa). The molecular weight of IL-12B (p40) is 34.6 kDa (306aa). The heterodimeric IL-12 has a predicted molecular weight of 57.9kDa
Molecular weight on SDS-PAGE will appear higher.
IL-12A (p35) RNLPVATPDPGMFPCLHHSQNLLRAVSNMLQKARQTLEFYPCTSEEIDHEDITKDKTSTVEACLPLELTKNESCLNSRETSFITNGSCLASRKTSFMMALCLSSIYEDLKMYQVEFKTMNAKLLMDPKRQIFLDQNMLAVIDELMQALNFNSETVPQKSSLEEPDFYKTKIKLCILLHAFRIRAVTIDRVMSYLNASHHHHHH
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: 10% Glycerol (glycerin, glycerine), 0.03% EDTA, 89% PBS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- CLMF p35
- CLMF p40
FunctionCytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC.
Sequence similaritiesBelongs to the IL-6 superfamily.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab187480 has not yet been referenced specifically in any publications.