Recombinant human IL-15 protein (ab214149)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 0.100 Eu/µg
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human IL-15 protein
See all IL-15 proteins and peptides -
Biological activity
Measured by its ability to stimulate the proliferation of mouse CTLL-2 cells. The ED50 for this effect is typically 0.5 ng/mL, corresponding to a specific activity of 2x 106 units/mg.
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Purity
> 95 % SDS-PAGE. -
Endotoxin level
< 0.100 Eu/µg -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
NWVNVISDLK KIEDLIQSMH IDATLYTESD VHPSCKVTAM KCFLLELQVI SLESGDASIH DTVENLIILA NNSLSSNGNV TESGCKECEE LEEKNIKEFL QSFVHIVQMF INTS -
Predicted molecular weight
14 kDa including tags -
Amino acids
49 to 162 -
Tags
His tag C-Terminus -
Additional sequence information
This product is for the mature full length protein. The signal peptide is not included.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab214149 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Constituent: 100% PBS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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Reconstitutionab214149 was lyophilized from 0.2 µm-filtered solution in PBS. Reconstitute 10 µg vial with 100 µl sterile water to a concentration of 0.1 mg/ml. Add 1X PBS to the desired protein concentration. PBS containing at least 0.1% BSA should be used for further dilutions.
General Info
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Alternative names
- IL 15
- IL-15
- IL15
see all -
Function
Cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL-15 requires interaction of IL-15 with components of IL-2R, including IL-2R beta and probably IL-2R gamma but not IL-2R alpha. -
Tissue specificity
Most abundant in placenta and skeletal muscle. It is also detected in the heart, lung, liver and kidney. IL15-S21AA is preferentially expressed in tissues such as testis and thymus. -
Sequence similarities
Belongs to the IL-15/IL-21 family. -
Cellular localization
Secreted and Cytoplasm. Nucleus. IL15-S21AA is not secreted, but rather is stored intracellularly, appearing in the nucleus and cytoplasmic components. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab214149 has not yet been referenced specifically in any publications.