Overview

Description

  • Nature

    Recombinant
  • Source

    HEK 293 cells
  • Amino Acid Sequence
    • Accession
    • Species

      Human
    • Sequence

      FTLQPAAHTGAARSCRFRGRHYKREFRLEGEPVALRCPQVPYWLWASVSP RINLTWHKNDSARTVPGEEETRMWAQDGALWLLPALQEDSGTYVCTTRNA SYCDKMSIELRVFENTDAFLPFISYPQILTLSTSGVLVCPDLSEFTRDKT DVKIQWYKDSLLLDKDNEKFLSVRGTTHLLVHDVALEDAGYYRCVLTFAH EGQQYNITRSIELRIKKKKEETIPVIISPLKTISASLGSRLTIPCKVFLG TGTPLTTMLWWTANDTHIESAYPGGRVTEGPRQEYSENNENYIEVPLIFD PVTREDLHMDFKCVVHNTLSFQTLRTTVKE
    • Molecular weight

      64 kDa including tags
    • Amino acids

      14 to 343
    • Additional sequence information

      Extracellular domain fused with a human IgG1 Fc tag (Pro 100 - Lys 330; P01857) at the C-terminus (NP_004624.1).

Associated products

Specifications

Our Abpromise guarantee covers the use of ab221219 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    Measured by its binding ability in a functional ELISA. Immobilized ab221219 at 10 μg/ml (100 µl/well), can bind Biotinylated Human IL-1 alpha with a linear range of 0.05-1 μg/ml.

  • Applications

    Functional Studies

    SDS-PAGE

  • Endotoxin level

    < 1.000 Eu/µg
  • Purity

    > 95 % SDS-PAGE.

  • Form

    Lyophilised
  • Additional notes

    Previously labelled as IL1 Receptor II.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 7.50
    Constituents: 0.61% Tris, 0.75% Glycine, 5% Trehalose

    Lyophilized from 0.22 µm filtered solution.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    It is recommended to reconstitute the lyophilized product in sterile deionized water to a final concentration of 1 mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.

General Info

  • Alternative names

    • IL1 Receptor II
    • Antigen CDw121b
    • CD121
    • CD121 antigen-like family member B
    • CD121b
    • CD121B Antigen
    • CDw121b
    • IL 1 type II receptor
    • IL-1R-2
    • IL-1R-beta
    • IL-1R2
    • IL-1RT-2
    • IL-1RT2
    • IL1R Beta
    • Il1r-2
    • IL1R2
    • IL1R2_HUMAN
    • IL1R2c
    • IL1RB
    • Interleukin 1 receptor type II variant 3
    • Interleukin 1 receptor, beta, type II
    • Interleukin 1 receptor, type II
    • Interleukin-1 receptor beta
    • Interleukin-1 receptor type 2
    • Interleukin-1 receptor type II
    • MGC137383
    • MGC47725
    • OTTHUMP00000207969
    • p68
    • Type II interleukin-1 receptor, beta
    see all
  • Function

    Receptor for interleukin-1 alpha (IL-1A), beta (IL-1B), and interleukin-1 receptor antagonist protein (IL-1ra).
  • Sequence similarities

    Belongs to the interleukin-1 receptor family.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
  • Cellular localization

    Membrane.
  • Information by UniProt

Images

  • SDS-PAGE analysis of reduced ab221219 stained overnight with Coomassie Blue.

    The protein migrates as 68-74 kDa on a SDS-PAGE gel under reducing conditions due to glycosylation.

  • Immobilized ab221219 at 10 μg/ml (100 µl/well), can bind Biotinylated Human IL-1 alpha with a linear range of 0.05-1 μg/ml.

References

ab221219 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab221219.
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