Overview

Description

  • Nature

    Recombinant
  • Source

    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species

      Human
    • Sequence

      The sequence of the first five N-terminal amino acids was determined and was found to be Met-Arg-Pro-Ser-Gly.
    • Molecular weight

      17 kDa
    • Amino acids

      26 to 177

Specifications

Our Abpromise guarantee covers the use of ab119745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    The ED50 as determined by the dose-dependent inhibition of IL1 stimulation of D10S cells was found to be 0.5 ng/ml.
  • Applications

    SDS-PAGE

    HPLC

    Functional Studies

  • Purity

    > 95 % SDS-PAGE.
    Purified by proprietary chromatographic techniques the purity was determined to be > 98 % by SDS-PAGE and RP-HPLC.
  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute in sterile ddH2O to a concentration >= 100 µg/ml.

General Info

  • Alternative names

    • DIRA
    • F630041P17Rik
    • ICIL 1RA
    • ICIL-1RA
    • ICIL1RA
    • IL-1ra
    • IL-1ra3
    • IL-1RN
    • IL1 inhibitor
    • IL1F3
    • IL1RA
    • IL1RA_HUMAN
    • IL1RN
    • IL1RN (IL1F3)
    • Interleukin 1 receptor antagonist
    • Interleukin-1 receptor antagonist protein
    • Intracellular IL 1 receptor antagonist type II
    • Intracellular interleukin 1 receptor antagonist (icIL 1ra)
    • IRAP
    • MGC10430
    • MVCD4
    • Type II interleukin 1 receptor antagonist
    see all
  • Function

    Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure.
  • Tissue specificity

    The intracellular form of IL1RN is predominantly expressed in epithelial cells.
  • Involvement in disease

    Microvascular complications of diabetes 4
    Interleukin 1 receptor antagonist deficiency
  • Cellular localization

    Cytoplasm and Secreted.
  • Information by UniProt

References

ab119745 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for contacting us. We generally ship these proteins overnight on an ice pack at 4C. The lyophilized protein ab119745 should be stable for longer shipment at ambient temperature, but the liquid protein may not be. I am currently checking with my colleague who coordinates our South American orders to check what shipping methods are used for Columbia. I will let you know as soon as I have any additional information.

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