Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies, WB
Product nameRecombinant human IL-1RA protein
See all IL-1RA proteins and peptides
Determined by its ability to inhibit the IL-1alpha stimulation of murine D10S cell. The expected ED50 is 20-40 ng/ml in the presence of 50 pg/ml of IL-1alpha.
Purity> 95 % SDS-PAGE.
ab50081 purity was assessed also by HPLC. Endotoxin level is less than 0.1 ng per µg (1EU/µg).
Expression systemEscherichia coli
Protein lengthFull length protein
SequenceMRPSGRKSSK MQAFRIWDVN QKTFYLRNNQ LVAGYLQGPN VNLEEKIDVV PIEPHALFLG IHGGKMCLSC VKSGDETRLQ LEAVNITDLS ENRKQDKRFA FIRSDSGPTT SFESAACPGW FLCTAMEADQ PVSLTNMPDE GVMVTKFYFQ EDE
Amino acids24 to 177
Our Abpromise guarantee covers the use of ab50081 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This protein is the 17.2 kDa secreted isoform of IL-1RA.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C long term.
Constituents: 0.0605% Tris, 0.145% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionReconstitute to 1mg/ml with distilled water.
- ICIL 1RA
FunctionInhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure.
Tissue specificityThe intracellular form of IL1RN is predominantly expressed in epithelial cells.
Involvement in diseaseMicrovascular complications of diabetes 4
Interleukin 1 receptor antagonist deficiency
Cellular localizationCytoplasm and Secreted.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab50081 has not yet been referenced specifically in any publications.