Recombinant human IL-2 protein (Active) (ab9619)
Key features and details
- Expression system: Escherichia coli
- Purity: >= 98% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: HPLC, Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human IL-2 protein (Active)
See all IL-2 proteins and peptides -
Biological activity
The ED50, as determined by the dose-dependent stimulation of murine CTLL-2 cells, is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.
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Purity
>= 98 % SDS-PAGE.
>=98% HPLC analyses. Sterile filtered. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MAPTSSSTKK TQLQLEHLLL DLQMILNGIN NYKNPKLTRM LTFKFYMPKK ATELKHLQCL EEELKPLEEV LNLAQSKNFH LRPRDLISNI NVIVLELKGS ETTFMCEYAD ETATIVEFLN RWITFAQSII STLT -
Predicted molecular weight
16 kDa -
Amino acids
21 to 153 -
Additional sequence information
Full length mature protein, without the signal peptide. This protein also includes an initiator methionine.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab9619 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
HPLC
Functional Studies
SDS-PAGE
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituent: 0.29% Sodium citrate
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionCentrifuge the vial prior to opening. Reconstitute in 100 mM Acetic Acid to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4oC for 1 week or -20oC for future use.
General Info
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Alternative names
- Aldesleukin
- IL 2
- IL-2
see all -
Function
Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. -
Involvement in disease
Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17. -
Sequence similarities
Belongs to the IL-2 family. -
Cellular localization
Secreted. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab9619 has not yet been referenced specifically in any publications.