Bioactive grade

Recombinant human IL-21 protein (ab214152)

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Description

  • Product name

    Recombinant human IL-21 protein
    See all IL-21 proteins and peptides

  • Biological activity

    Measured by a cell proliferation assay using Human N1186 T cells. The ED50 for this effect is typically 10 ng/mL, corresponding to a specific activity of 1x 105 units/mg.

  • Purity

    >= 95 % SDS-PAGE.

  • Endotoxin level

    < 0.010 Eu/µg

  • Expression system

    HEK 293 cells

  • Accession

    NP_068575.1

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      QDRHMIRMR QLIDIVDQLK NYVNDLVPEF LPAPEDVETN CEWSAFSCFQ KAQLKSANTG NNERIINVSI KKLKRKPPST NAGRRQKHRL TCPSCDSYEK KPPKEFLERF KSLLQKMIHQ HLSSRTHGSE DS

    • Predicted molecular weight

      18 kDa including tags

    • Amino acids

      32 to 162

    • Tags

      His tag C-Terminus

Specifications

Our Abpromise guarantee covers the use of ab214152 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

    Constituent: 100% PBS

    Lyophilized from 0.2 µm filtered solution.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute 10 µg vial with 100 µl sterile water to a concentration of 0.1 mg/ml. PBS containing at least 0.1% BSA should be used for further dilutions.

General Info

  • Alternative names

    • CVID11
    • IL 21
    • IL-21
    • Il21
    • IL21_HUMAN
    • Interleukin 21
    • Interleukin-21
    • interleukin-21 isoform
    • Interleukin21
    • OTTHUMP00000164088
    • Za11
    see all

  • Function

    Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells (By similarity). May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation.

  • Tissue specificity

    Expressed in activated CD4-positive T-cells but not in CD8-positive T-cells, B-cells, or monocytes.

  • Sequence similarities

    Belongs to the IL-15/IL-21 family.

  • Cellular localization

    Secreted.
  • Information by UniProt

References

ab214152 has not yet been referenced specifically in any publications.

Publishing research using ab214152? Please let us know so that we can cite the reference in this datasheet.

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