Recombinant human IL-21 protein (ab214152)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Endotoxin level: < 0.010 Eu/µg
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Recombinant human IL-21 protein
See all IL-21 proteins and peptides -
Biological activity
Measured by a cell proliferation assay using Human N1186 T cells. The ED50 for this effect is typically 10 ng/mL, corresponding to a specific activity of 1x 105 units/mg.
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Purity
>= 95 % SDS-PAGE. -
Endotoxin level
< 0.010 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
QDRHMIRMR QLIDIVDQLK NYVNDLVPEF LPAPEDVETN CEWSAFSCFQ KAQLKSANTG NNERIINVSI KKLKRKPPST NAGRRQKHRL TCPSCDSYEK KPPKEFLERF KSLLQKMIHQ HLSSRTHGSE DS -
Predicted molecular weight
18 kDa including tags -
Amino acids
32 to 162 -
Tags
His tag C-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab214152 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Constituent: 100% PBS
Lyophilized from 0.2 µm filtered solution.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute 10 µg vial with 100 µl sterile water to a concentration of 0.1 mg/ml. PBS containing at least 0.1% BSA should be used for further dilutions.
General Info
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Alternative names
- CVID11
- IL 21
- IL-21
see all -
Function
Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells (By similarity). May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation. -
Tissue specificity
Expressed in activated CD4-positive T-cells but not in CD8-positive T-cells, B-cells, or monocytes. -
Sequence similarities
Belongs to the IL-15/IL-21 family. -
Cellular localization
Secreted. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab214152 has not yet been referenced specifically in any publications.