Key features and details
- Expression system: HEK 293 cells
- Purity: > 90% SDS-PAGE
- Endotoxin level: < 0.060 Eu/µg
- Active: Yes
- Tags: DDDDK tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant human IL-23 protein (Active)
See all IL-23 proteins and peptides
Induction of IL-17 secretion by mouse splenocytes.
Purity> 90 % SDS-PAGE.
Endotoxin level< 0.060 Eu/µg
Expression systemHEK 293 cells
Protein lengthFull length protein
SequenceIWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCSGSTS GSGKPGSGEG STKGRAVPGG SSPAWTQCQQ LSQKLCTLAW SAHPLVGHMD LREEGDEETT NDVPHIQCGD GCDPQGLRDN SQFCLQRIHQ GLIFYEKLLG SDIFTGEPSL LPDSPVGQLH ASLLGLSQLL QPEGHHWETQ QIPSLSPSQP WQRLLLRFKI LRSLQAFVAV AARVFAHGAA TLSP
Predicted molecular weight70 kDa including tags
Amino acids23 to 328
TagsDDDDK tag N-Terminus
Additional sequence informationFused at the C-terminus to a 18 aa linker peptide (GSTSGSGKPGSGEGSTKG) and to the IL23 p19 peptide (aa 20-189) and tagged at the N-terminus to a DDDDK-tag. IL-12p40 (human) Swissprot: P29460
- Anti-IL-23 antibody [HLT2736] (ab110630)
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1162)
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (ab18230)
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab21536)
- Anti-IL-23 antibody (ab45420)
- Anti-IL-23 antibody [G23-8] (ab91271)
Our Abpromise guarantee covers the use of ab109153 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Induction of IL17 secretion by Mouse splenocytes.
PBS containing at least 0.1% BSA should be used for further dilutions.
Reconstitute 10µg vial with 100 µl sterile water to a concentration of 0.1mg/ml.
Add 1X PBS to the desired protein concentration.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Please see notes section.
Constituent: 99% PBS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionReconstitute 10µg vial with 100 µl sterile water to a concentration of 0.1mg/ml. Add 1X PBS to the desired protein concentration.
- IL 23
- IL 23 A
- IL 23 subunit alpha
FunctionAssociates with IL12B to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to an heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
Tissue specificitySecreted by activated dendritic and phagocytic cells and keratinocytes. Also expressed by dermal Langerhans cells (at protein level).
Sequence similaritiesBelongs to the IL-6 superfamily.
Developmental stageExpressed by newborns dendritic cells.
Cellular localizationSecreted. Secreted upon association with IL12B.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab109153 has not yet been referenced specifically in any publications.