Description

  • Product name

    Recombinant human IL-23 protein (Active)
    See all IL-23 proteins and peptides
  • Biological activity

    Induction of IL-17 secretion by mouse splenocytes.

  • Purity

    > 90 % SDS-PAGE.

  • Endotoxin level

    < 0.060 Eu/µg
  • Expression system

    HEK 293 cells
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCSGSTS GSGKPGSGEG STKGRAVPGG SSPAWTQCQQ LSQKLCTLAW SAHPLVGHMD LREEGDEETT NDVPHIQCGD GCDPQGLRDN SQFCLQRIHQ GLIFYEKLLG SDIFTGEPSL LPDSPVGQLH ASLLGLSQLL QPEGHHWETQ QIPSLSPSQP WQRLLLRFKI LRSLQAFVAV AARVFAHGAA TLSP
    • Predicted molecular weight

      70 kDa including tags
    • Amino acids

      23 to 328
    • Tags

      DDDDK tag N-Terminus
    • Additional sequence information

      Fused at the C-terminus to a 18 aa linker peptide (GSTSGSGKPGSGEGSTKG) and to the IL23 p19 peptide (aa 20-189) and tagged at the N-terminus to a DDDDK-tag. IL-12p40 (human) Swissprot: P29460

Specifications

Our Abpromise guarantee covers the use of ab109153 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Lyophilised
  • Additional notes

    Induction of IL17 secretion by Mouse splenocytes.

    Stable for at least 1 year after receipt when stored at -20°C.
    Working aliquots are stable for up to 3 months when stored at -20°C.


    PBS containing at least 0.1% BSA should be used for further dilutions.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

    Constituent: 99% PBS

    0.2µm-filtered.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute 10µg vial with 100 µl sterile water to a concentration of 0.1mg/ml. Add 1X PBS to the desired protein concentration.

General Info

  • Alternative names

    • IL 23
    • IL 23 A
    • IL 23 subunit alpha
    • IL 23A
    • IL 23p19
    • IL-23 subunit alpha
    • IL-23-A
    • IL-23p19
    • IL12B
    • IL23
    • Il23a
    • IL23A_HUMAN
    • IL23P19
    • interleukin 12B
    • Interleukin 23 alpha subunit p19
    • Interleukin 23 p19 subunit
    • interleukin 23 subunit alpha
    • interleukin 23 subunit p19
    • interleukin six, G CSF related factor
    • Interleukin-23 subunit alpha
    • Interleukin-23 subunit p19
    • JKA3 induced upon T cell activation
    • MGC79388
    • P19
    • SGRF
    see all
  • Function

    Associates with IL12B to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to an heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
  • Tissue specificity

    Secreted by activated dendritic and phagocytic cells and keratinocytes. Also expressed by dermal Langerhans cells (at protein level).
  • Sequence similarities

    Belongs to the IL-6 superfamily.
  • Developmental stage

    Expressed by newborns dendritic cells.
  • Cellular localization

    Secreted. Secreted upon association with IL12B.
  • Information by UniProt

Images

  • IL17 secretion by Mouse splenocytes
    Mononuclear cells isolated from Mouse spleen were cultured with Mouse IL23 in presence or absence of various concentrations of ab109153 for 3 days. Levels of IL17 accumulated in culture supernatants were measured using ELISA.

References

ab109153 has not yet been referenced specifically in any publications.

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