Recombinant human IL-27-A + EBI3 protein (Fc Chimera Active) (ab214982)
Key features and details
- Expression system: CHO cells
- Purity: >= 98% SDS-PAGE
- Endotoxin level: < 0.060 Eu/µg
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human IL-27-A + EBI3 protein (Fc Chimera Active) -
Biological activity
Bioactivity was measured in a cell proliferation assay using anti-CD3 activated human peripheral mononuclear cells.
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Purity
>= 98 % SDS-PAGE. -
Endotoxin level
< 0.060 Eu/µg -
Expression system
CHO cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Amino Acid Sequence 1
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Species
Human -
Sequence
VWGFPRPPGRPQLSLQELRREFTVSLHLARKLLSEVRGQAHRFAESHLPG VNLYLLPLGEQLPDVSLTFQAWRRLSDPERLCFISTTLQPFHALLGGLGT QGRWTNMERMQLWAMRLDLRDLQRHLRFQVLAAGFNLPEEEEEEEEEEEE ERKGLLPGALGSALQGPAQVSWPQLLSTYRLLHSLELVLSRAVRELLLLS KAGHSVWPLGFPTLSPQP -
Amino acids
26 to 243 -
Additional sequence information
Mature protein without signal peptide fused through a polypeptide linker to the Fc portion of a mutant Human IgG1.
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Amino Acid Sequence 2
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Species
Human -
Sequence
RKGPPAALTLPRVQCRASRYPIAVDCSWTLPPAPNSTSPVSFIATYRLGM AARGHSWPCLQQTPTSTSCTITDVQLFSMAPYVLNVTAVHPWGSSSSFVP FITEHIIKPDPPEGVRLSPLAERQLQVQWEPPGSWPFPEIFSLKYWIRYK RQGAARFHRVGPIEATSFILRAVRPRARYYVQVAAQDLTDYGELSDWSLP ATATMSLGK -
Amino acids
21 to 229 -
Additional sequence information
Mature protein without signal peptide fused through a polypeptide linker to the Fc portion of a mutant human IgG1.
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Specifications
Our Abpromise guarantee covers the use of ab214982 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Lyophilized -
Additional notes
Non-lytic: Acts as a long lasting fusion protein which only binds to the receptor. Mutations to the complement (C1q) and FcgR I binding sites of the IgGs Fc fragment render the fusion proteins incapable of antibody directed cytotoxicity (ADCC) and complement directed cytotoxicity (CDC).
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Constituent: 100% PBS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute in 50 µL sterile water. Add 1X PBS to the desired protein concentration. Working aliquots are stable for up to 3 months when stored at -20°C.
General Info
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Alternative names
- IL27-A+EBI3
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Cellular localization
IL27-A: Secreted. Does not seem to be secreted without coexpression of EBI3. EBI3: Secreted.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab214982 has not yet been referenced specifically in any publications.