Key features and details
- Expression system: Escherichia coli
- Purity: > 97% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: Functional Studies, HPLC, SDS-PAGE
Product nameRecombinant human IL-3 protein
See all IL-3 proteins and peptides
Fully biologically active when compared to standard.
The ED50 as determined by the dose-dependant stimulation of the proliferation of Human TF-1 cells is less than 0.1 ng/mL, corresponding to a Specific Activity of 1.0 x 107 IU/mg.
Purity> 97 % SDS-PAGE.
> 97 % by HPLC analysis.
Endotoxin level< 1.000 Eu/µg
Expression systemEscherichia coli
Protein lengthFull length protein
SequenceAPMTQTTSLKTSWVNCSNMIDEIITHLKQPPLPLLDFNNLNGEDQDILME NNLRRPNLEAFNRAVKSLQNASAIESILKNLLPCLPLATAAPTRHPIHIK DGDWNEFRRKLTFYLKTLENAQAQQTTLSLAIF
Predicted molecular weight15 kDa
Amino acids20 to 152
Additional sequence informationSingle non-glycosylated polypeptide chain. This product is the mature full length protein from aa 20-152. The signal peptide is not included.
Our Abpromise guarantee covers the use of ab200297 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle.
Constituent: 100% PBS
Lyophilized from a 0.2 µM filtered solution.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionBriefly centrifuge vial prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20°C to -70°C. Further dilutions should be made in appropriate buffered solutions.
- Colony stimulating factor multiple
- Hematopoietic growth factor
- IL 3
FunctionGranulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.
This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.
Tissue specificityActivated T-cells, mast cells, natural killer cells.
Sequence similaritiesBelongs to the IL-3 family.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab200297 has not yet been referenced specifically in any publications.