Bioactive grade

Recombinant human IL-3 protein (ab200297)

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Description

  • Product name

    Recombinant human IL-3 protein
    See all IL-3 proteins and peptides

  • Biological activity

    Fully biologically active when compared to standard.

    The ED50 as determined by the dose-dependant stimulation of the proliferation of Human TF-1 cells is less than 0.1 ng/mL, corresponding to a Specific Activity of 1.0 x 107 IU/mg.

  • Purity

    > 97 % SDS-PAGE.
    > 97 % by HPLC analysis.

  • Endotoxin level

    < 1.000 Eu/µg

  • Expression system

    Escherichia coli

  • Accession

    P08700

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      APMTQTTSLKTSWVNCSNMIDEIITHLKQPPLPLLDFNNLNGEDQDILME NNLRRPNLEAFNRAVKSLQNASAIESILKNLLPCLPLATAAPTRHPIHIK DGDWNEFRRKLTFYLKTLENAQAQQTTLSLAIF

    • Predicted molecular weight

      15 kDa

    • Amino acids

      20 to 152

    • Additional sequence information

      Single non-glycosylated polypeptide chain. This product is the mature full length protein from aa 20-152. The signal peptide is not included.

Associated products

Specifications

Our Abpromise guarantee covers the use of ab200297 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    HPLC

    SDS-PAGE

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle.

    pH: 7.40
    Constituent: 100% PBS

    Lyophilized from a 0.2 µM filtered solution.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Briefly centrifuge vial prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20°C to -70°C. Further dilutions should be made in appropriate buffered solutions.

General Info

  • Alternative names

    • Colony stimulating factor multiple
    • Hematopoietic growth factor
    • IL 3
    • IL-3
    • IL3
    • IL3_HUMAN
    • Interleukin 3
    • Interleukin 3 (colony stimulating factor, multiple)
    • Interleukin-3
    • Mast cell growth factor
    • MCGF
    • MGC79398
    • MGC79399
    • Multi CSF
    • Multilineage colony stimulating factor
    • Multipotential colony stimulating factor
    • Multipotential colony-stimulating factor
    • OTTHUMP00000065963
    • P cell stimulating factor
    • P-cell-stimulating factor
    see all

  • Function

    Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.
    This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.

  • Tissue specificity

    Activated T-cells, mast cells, natural killer cells.

  • Sequence similarities

    Belongs to the IL-3 family.

  • Cellular localization

    Secreted.
  • Information by UniProt

References

ab200297 has not yet been referenced specifically in any publications.

Publishing research using ab200297? Please let us know so that we can cite the reference in this datasheet.

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