Product nameRecombinant human IL-3 protein
See all IL-3 proteins and peptides
Protein lengthFull length protein
SourceHEK 293 cells
Amino Acid Sequence
SequenceTheoretical Sequence: APMTQTTSLKTSWVNCSNMIDEIITHLKQPPLPLLDFNNLNGEDQDILME NNLRRPN LEAFNRAVKSLQNASAIESILKNLLPCLPLATAAPTRHPIH IKDGDWNEFRRKLTFYL KTLENAQAQQTTLSLAIF
Our Abpromise guarantee covers the use of ab83685 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activityActivity: The ED50 of ab83685 is typically 0.1- 0.4 ng/ml as measured using the human growth factor dependent TF1 cell line.
Purity> 95 % SDS-PAGE.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C.
Constituents: PBS, 1% Human serum albumin, 10% Trehalose
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionIt is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
- Colony stimulating factor multiple
- Hematopoietic growth factor
- IL 3
FunctionGranulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.
This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.
Tissue specificityActivated T-cells, mast cells, natural killer cells.
Sequence similaritiesBelongs to the IL-3 family.
- Information by UniProt
Densitometry of protein isoforms visualised by 2-DE. The triangle indicates the theoretical MW and pI of the protein.
1D gel data:
Lane 1 ab83685
Lane 2 ab83685 treated with PNGase F to remove potential N-linked glycans
Lane 4 ab83685 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.
5 μg protein loaded per lane; Deep Purple™ stained. Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. Faint bands in lane 2 and lane 3 are glycosidase enzymes.
2D gel data:
A sample of ab83685 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. 40 μg protein loaded per lane; Deep Purple™ stained.
Spot train indicates presence of multiple isoforms of ab83685.
Spots within the spot train were cut from the gel and identified as IL-3 by protein mass fingerprinting.
ab83685 has not yet been referenced specifically in any publications.