Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
- High batch-to-batch consistency
- Optimal bioactivity
- Guaranteed identical to human native proteins
- >95% purity
- Ultra-low endotoxin levels: <0.005 Eu/µg
- Carrier and tag free
Product nameRecombinant human IL-3 protein
See all IL-3 proteins and peptides
Activity: The ED50 of ab83685 is typically 0.1- 0.4 ng/ml as measured using the human growth factor dependent TF1 cell line.
Purity> 95 % SDS-PAGE.
Expression systemHEK 293 cells
Protein lengthFull length protein
SequenceTheoretical Sequence: APMTQTTSLKTSWVNCSNMIDEIITHLKQPPLPLLDFNNLNGEDQDILME NNLRRPN LEAFNRAVKSLQNASAIESILKNLLPCLPLATAAPTRHPIH IKDGDWNEFRRKLTFYL KTLENAQAQQTTLSLAIF
Our Abpromise guarantee covers the use of ab83685 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C.
Constituents: PBS, 1% Human serum albumin, 10% Trehalose
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionIt is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
- Colony stimulating factor multiple
- Hematopoietic growth factor
- IL 3
FunctionGranulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.
This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.
Tissue specificityActivated T-cells, mast cells, natural killer cells.
Sequence similaritiesBelongs to the IL-3 family.
- Information by UniProt
Densitometry of protein isoforms visualised by 2-DE. The triangle indicates the theoretical MW and pI of the protein.
1D gel data:
Lane 1 ab83685
Lane 2 ab83685 treated with PNGase F to remove potential N-linked glycans
Lane 4 ab83685 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.
5 μg protein loaded per lane; Deep Purple™ stained. Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. Faint bands in lane 2 and lane 3 are glycosidase enzymes.
2D gel data:
A sample of ab83685 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. 40 μg protein loaded per lane; Deep Purple™ stained.
Spot train indicates presence of multiple isoforms of ab83685.
Spots within the spot train were cut from the gel and identified as IL-3 by protein mass fingerprinting.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab83685 has not yet been referenced specifically in any publications.